Analytical Data
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Gene name
HSD17b3
- Application
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Alternative Names
SDR12C2; EDH17B3; Short Chain Dehydrogenase/Reductase Family 12C,Member 2; Testosterone 17-Beta-Dehydrogenase 3; Testicular 17-beta-hydroxysteroid dehydrogenase
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Species
Human
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Source
E. coli
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Tag
N-His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P37058
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Expression Region
Met1~Arg310
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Molecular Weight
33kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
HSD17B3, or 17β-hydroxysteroid dehydrogenase type 3, is a key enzyme in the biosynthesis of androgens, specifically testosterone, from dehydroepiandrosterone (DHEA). This enzyme plays a critical role in the regulation of steroid hormone levels and is essential for normal sexual development and reproductive function. Mutations in the HSD17B3 gene can lead to disorders of sexual development, such as androgen insensitivity syndrome and male pseudohermaphroditism, highlighting its importance in clinical endocrinology. The study of recombinant HSD17B3 protein is crucial for understanding its biochemical properties, role in steroidogenesis, and potential therapeutic applications. Utilizing recombinant DNA technology, scientists can produce HSD17B3 in vitro, allowing for detailed analysis of its structure, function, and interaction with substrates and inhibitors. This research not only provides insight into the enzyme's mechanisms but also aids in the development of targeted treatments for disorders associated with steroid hormone imbalance. By elucidating the functional characteristics of HSD17B3 through recombinant protein studies, researchers aim to pave the way for novel strategies in endocrinology and reproductive medicine, thus contributing to improved patient outcomes and advancing our understanding of steroid metabolism.
