Analytical Data
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Gene name
AQP4
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简介
The AQP4 protein forms water-specific channels and is involved in brain water balance and solute transport. AQP4 Protein, Human (His) is the recombinant human-derived AQP4, expressed by E. coli, with N-6*His labeled tag. The total length of AQP4 Protein, Human (His) is 71 a.a..
- Application
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Alternative Names
AQP 4; AQP-4; AQP4; AQP4_HUMAN; Aquaporin type 4; Aquaporin-4; Aquaporin4; HMIWC 2; HMIWC2; Mercurial insensitive water channel; Mercurial-insensitive water channel; MGC22454; MIWC; WCH 4; WCH4
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Species
Human
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Source
E. coli
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Tag
N-6*His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P55087-1
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Expression Region
C253-V323
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Protein Length
Partial
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Molecular Weight
16 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Aquaporin-4 (AQP4) is a pivotal water channel protein predominantly expressed in the brain, playing a vital role in maintaining water homeostasis and regulating osmotic pressure in neural tissues. Its importance has been highlighted in various physiological processes, including cerebral edema, brain development, and neuronal signaling. Research into AQP4 has gained momentum due to its implications in several neurological disorders, such as Alzheimer’s disease, multiple sclerosis, and neuromyelitis optica, underscoring the need for a deeper understanding of its structure and function. Recombinant AQP4 proteins have become invaluable tools for investigating the channel's specific roles in cellular and molecular contexts, allowing for detailed studies on its biophysical properties and interactions with other cellular components. Advances in recombinant DNA technology have enabled the production of large quantities of AQP4, facilitating high-throughput screening and biophysical assays that were previously challenging. Furthermore, elucidating the mechanisms governing AQP4's functional dynamics can provide insights into therapeutic targets for diseases characterized by dysregulated water transport. By exploring the structure-function relationship of AQP4 through recombinant approaches, researchers aim to develop novel strategies for treating conditions linked to water imbalance in the central nervous system. The ongoing studies on AQP4 not only enhance our knowledge of its fundamental biology but also pave the way for innovative therapeutic interventions targeted at improving neurological health. This growing field of research highlights the significance of AQP4 not only as a water channel but as a crucial player in brain pathophysiology, marking it as a key focus for future neuroscientific investigations.
