Analytical Data
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Gene name
VE-Cadherin
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简介
VE-cadherin is critical for cellular functions such as β-catenin binding and signaling receptor activity, and is involved in processes such as cell-cell junction organization and vascular maturation. It is predicted to localize in cell-cell junctions and nuclei, serving as a biomarker for pulmonary hypertension. VE-Cadherin Protein, Rat (HEK293, His) is the recombinant rat-derived VE-Cadherin protein, expressed by HEK293 , with C-His labeled tag.
- Application
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Biological Activity
Measured by the ability of the immobilized protein to support the adhesion of HUVEC cells. The ED50 for this effect is 11.93 ng/mL. Corresponding to a specific activity is 8.382×104 units/mg. Measured by the ability of the immobilized protein to support the adhesion of HUVEC cells. The ED50 for this effect is 11.93 ng/mL. Corresponding to a specific activity is 8.382×104units/mg.
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Alternative Names
Cadherin-5; VE-cadherin; CD144; CDH5
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Species
Rat
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Source
HEK293
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Tag
C-His
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Purity
Greater than 95% as determined by SDS-PAGE.
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Uniprot
NP_001100877
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Expression Region
G25-Q585
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AA Sequence
GPNMLPVHQRQKRDWIWNQMHIDEEKNESLPHYVGKIKSNVNRQNAKYVLQGEQAGKIFRVDADTGDVLAYERLDREKVSEYFLTALIVDKKTNKNLEQPSSFTVKVHDVNDNWPVFTHQVFNASVPEMSAIGTSVIRVTATDADDPTVAGHATVLYQMVKGNEYFAIDNSGLIFTKDKNLDRETRAEYKIVVEAQDAQGLRGESGTATVLIRLEDINDNFPIFTQSTYTFSVPEDIRVGKPLGSLSVEDPDEPQNRMTKYSIMQGEYRDTFTIETDPDRNEGIIKPTKPLDYELIQQYTFHIEATDPTIRLGYLSSTAGKNKAKIIINVLDVDEPPVFQRRFYHFHLPENKKKPLIGTVVAKDPDKAQRSIGYSIRKTSDRGQYFRITKQGNIYNEKELDRETHAWYNLTVEANELDSRGNPVGKESIVQIYIEVLDENDNAPEFAQPYEPKVCENAAQGKLVVQISATDKDVVPVNTKFKFALKNEDSNFTLINNHDNTANITVKYGQFNREHTKFHYLPVLISDNGMPSLTGTGTLTVAVCKCNEQGEFTFCEEMAAQ
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Protein Length
Partial
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Molecular Weight
80-95 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
VE-Cadherin, or vascular endothelial cadherin, is a critical adhesion molecule primarily expressed in endothelial cells that form the inner lining of blood vessels. It plays a pivotal role in maintaining vascular integrity, regulating cell-cell junctions, and influencing various biological processes such as angiogenesis and inflammation. Given its key function in maintaining endothelial barrier stability, VE-Cadherin has garnered significant attention in the context of various pathological conditions, including cancer metastasis, cardiovascular diseases, and inflammatory disorders. Research into recombinant VE-Cadherin proteins has advanced our understanding of its structural and functional properties, providing tools for studying cell adhesion dynamics and signaling pathways. The generation of recombinant VE-Cadherin allows for the elucidation of its role in endothelial cell behavior and interactions, facilitating the development of therapeutic strategies aimed at modulating vascular functionality. Furthermore, recombinant forms of VE-Cadherin can serve as potential biomarkers or therapeutic targets, given their involvement in disease progression. With ongoing advancements in protein engineering and molecular biology, the study of VE-Cadherin continues to unveil insights into the complexities of endothelial biology and its implications in health and disease.
