Analytical Data
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Gene name
AGO2/Argonaute-2
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简介
The AGO2/Argonaute-2 protein is critical for RNA-mediated silencing complex (RISC)-mediated gene silencing. In "minimal RISC," AGO2 binds to short guide RNAs, such as microRNAs (miRNAs) or short interfering RNAs (siRNAs), to guide RISC for silencing with complementary mRNAs. AGO2/Argonaute-2 Protein, Mouse (sf9, His, solution) is the recombinant mouse-derived AGO2/Argonaute-2 protein, expressed by Sf9 insect cells , with N-His labeled tag.
- Application
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Alternative Names
Protein argonaute-2; Argonaute2; mAgo2; eIF-2C 2; Eif2c2
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Species
Mouse
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Source
Baculovirus
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Tag
N-His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q8CJG0
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Expression Region
M1-A860
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Protein Length
Full Length
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Molecular Weight
105 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Ago2, or Argonaute-2, is a critical protein in the RNA interference (RNAi) pathway, playing a pivotal role in gene regulation and post-transcriptional silencing of messenger RNA (mRNA). As a central component of the RNA-induced silencing complex (RISC), Ago2 binds to small RNA molecules, such as microRNAs (miRNAs) and small interfering RNAs (siRNAs), facilitating their targeting to complementary mRNA sequences. This interaction ultimately leads to mRNA degradation or translational repression, making Ago2 essential for maintaining cellular homeostasis and influencing various biological processes, including development, cell differentiation, and defense against viral infections. Research on Ago2 has gained significant interest due to its implications in cancer biology and therapeutic potential, as aberrations in RNAi pathways are frequently associated with tumorigenesis and disease progression. Moreover, Ago2 is considered a promising target for innovative gene-editing tools and applications in synthetic biology. Understanding the structure, function, and regulatory mechanisms of Ago2 can provide valuable insights into the intricacies of gene expression control and may lead to novel strategies for disease intervention. Thus, the study of Ago2 and its associated pathways remains a dynamic and crucial area of investigation within molecular biology and therapeutic development.
