Analytical Data
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Gene name
HLA-A*11:01&B2M&LMP2(SSCSSCPLTK) Tetramer
- Application
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Alternative Names
MHC; RMF; LMP2; LMP-2; Macropain chain 7; Proteasome chain 7; PSMB9; RING12
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Species
Human
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Source
HEK293
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Tag
C-Avi;C-8*His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
AAV53343.1 (G25-T305)&P61769
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Expression Region
AAV53343.1 (G25-T305)&P61769 (I21-M119)&SSCSSCPLTK
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Molecular Weight
260-265 kDa under Non reducing (N)
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
HLA-A*11:01, a major histocompatibility complex class I molecule, plays a crucial role in the immune response by presenting endogenous peptides to CD8+ T cells. Its association with various diseases, particularly viral infections and autoimmune disorders, highlights the importance of studying its peptide presentation capabilities. The beta-2 microglobulin (B2M) protein is essential for the proper folding and surface expression of HLA molecules, making it a critical component in the study of HLA-A*11:01. Additionally, LMP2, a component of the proteasome, is involved in the generation of peptides that bind to HLA molecules, including the specific peptide SSCSSCPLTK. This tetramer complex, composed of HLA-A*11:01, B2M, and the LMP2-derived peptide, serves as an invaluable tool for tracking and analyzing CD8+ T cell responses in various contexts, such as vaccine development and cancer immunotherapy. By utilizing this recombinant protein, researchers aim to gain deeper insights into T cell recognition, specificity, and activation mechanisms, ultimately contributing to the development of targeted immunotherapies and enhancing our understanding of immune regulation. The ongoing research surrounding this tetramer not only elucidates the roles of HLA molecules in immunity but also provides potential biomarkers for disease monitoring and therapeutic strategies.
