Analytical Data
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Gene name
SIRP beta 2
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简介
SIRP beta 2 Protein, a novel positive regulator of innate immunotherapy, is a member of the signal-regulatory-protein (SIRP) family, and also belongs to the immunoglobulin superfamily. SIRP beta 2 is expressed on myeloid cells at protein level and enhanced adhesion, differentiation, cancer cell phagocytosis and T cell activation in an in vitro setting. DAP12 is responsible for SIRP beta 2 signaling, and is recruited through a single, positive lysine residue in the transmembrane domain of SIRP beta 2. SIRP beta 2 Protein, Human (HEK293, Fc) is the recombinant human-derived SIRP beta 2 protein, expressed by HEK293 , with C-hFc labeled tag.
- Application
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Alternative Names
dJ776F14.2; PTPN1L; PTPNS1L3; Signal-Regulatory Protein Beta 2; Signal-Regulatory Protein Beta-2; SIRP beta 2; SIRP-beta-2; SIRPG
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Species
Human
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Source
HEK293
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Tag
C-hFc
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q5JXA9-1
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Expression Region
Q33-G287
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AA Sequence
QSSRNDWQVLQPEGPMLVAEGETLLLRCMVVGSCTDGMIKWVKVSTQDQQEIYNFKRGSFPGVMPMIQRTSEPLNCDYSIYIHNVTREHTGTYHCVRFDGLSEHSEMKSDEGTSVLVKGAGDPEPDLWIIQPQELVLGTTGDTVFLNCTVLGDGPPGPIRWFQGAGLSREAIYNFGGISHPKETAVQASNNDFSILLQNVSSEDAGTYYCVKFQRKPNRQYLSGQGTSLKVKAKSTSSKEAEFTSEPATEMSPTG
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Protein Length
Extracellular Domain
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Molecular Weight
80-95 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
SIRPβ2, a member of the Signal Regulatory Protein (SIRP) family, plays a crucial role in modulating immune responses and cellular signaling. This family of proteins, predominantly expressed in immune cells, interacts with CD47, a "don't eat me" signal recognized by macrophages, thereby influencing phagocytosis and immune evasion of various cells, including tumors. The beta 2 isoform has garnered attention due to its distinct functional properties compared to other SIRP family members, particularly its potential implications in cancer immunotherapy. Research indicates that SIRPβ2 may serve as an inhibitory receptor in immune signaling pathways, thus contributing to the regulation of T cell activation and macrophage function. Understanding the structural characteristics and functional mechanisms of SIRPβ2, particularly through the study of its recombinant protein, is essential for elucidating its role in immune modulation. The development of SIRPβ2 recombinant proteins aims to facilitate investigations into its interactions and downstream effects, paving the way for targeted therapeutic strategies that could enhance anti-tumor immunity and improve outcomes in cancer treatment. This research holds promise for the future of immunotherapy, as harnessing the modulation of SIRPβ2 may lead to innovative approaches in overcoming tumor resistance and promoting effective immune responses against malignancies.
