Analytical Data
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Gene name
DNTT
- Application
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Alternative Names
Terminal addition enzymeTerminal deoxynucleotidyltransferase
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Species
Human
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Source
E. coli
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Tag
N- His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P04053
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Expression Region
1-509aa
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Molecular Weight
62.5 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
DNTT, or Deoxynucleotidyltransferase, is an enzyme that plays a crucial role in DNA synthesis and repair. Research into the recombinant form of DNTT has gained significance due to its potential applications in genetic engineering, molecular biology, and therapeutic development. Traditionally, DNTT is sourced from biological systems, which can present challenges regarding yield, purity, and scalability. The recombinant production of DNTT allows for enhanced accessibility and application in various scientific fields. By utilizing recombinant DNA technology, researchers can express the DNTT gene in host organisms, such as bacteria or yeast, enabling the production of large quantities of the enzyme. This approach not only improves the efficiency of obtaining DNTT but also allows for post-translational modifications that can enhance its activity and stability. The purified recombinant DNTT enzyme is increasingly used in applications like gene therapy, where precise DNA manipulation is crucial, as well as in diagnostics and research settings where DNA synthesis is needed. Current studies focus on optimizing expression systems, improving enzyme characteristics, and exploring new applications, solidifying the importance of recombinant DNTT in modern biotechnology. As the demand for efficient and reliable DNA manipulation techniques continues to grow, the research and development of recombinant DNTT are expected to play a pivotal role in advancing genomic research and therapeutic strategies.
