Analytical Data
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Gene name
IRGM
- Application
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Alternative Names
Immunity-related GTPase family M protein 1 Interferon-inducible protein 1 LPS-stimulated RAW 264.7 macrophage protein 47 homolog
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Species
Human
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Source
E. coli
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Tag
N- GST
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
A1A4Y4
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Expression Region
1-181aa
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Molecular Weight
47.1 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
IRGM (Immunity Related GTPase M) is a crucial protein involved in the immune response and autophagy regulation, specifically in the context of host defense against intracellular pathogens. Research into IRGM has intensified due to its association with various diseases, including Crohn's disease and tuberculosis. Mutations and polymorphisms in the IRGM gene can influence susceptibility to these conditions by affecting the protein's function in the immune system. IRGM’s role in autophagy—where it aids in the degradation of pathogens—highlights its importance in maintaining cellular homeostasis. As the understanding of IRGM’s mechanisms deepens, scientists have increasingly focused on developing recombinant IRGM proteins for experimental applications. These studies aim to explore its structure, function, and interactions with other cellular components. By utilizing recombinant DNA technologies, researchers can produce IRGM in controlled laboratory settings, facilitating detailed biochemical analyses and functional assays. This work is essential for elucidating the pathways regulated by IRGM and for developing potential therapeutic strategies that target its pathways in diseases where it plays a significant role. Overall, the investigation of recombinant IRGM proteins is a pivotal aspect of current immunology research, with implications for understanding disease mechanisms and advancing therapeutic interventions.
