Analytical Data
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Gene name
MSH6
- Application
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Alternative Names
G/T mismatch-binding protein ;GTBP ;GTMBPMutS-alpha 160KDA subunit ;p160
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Species
Human
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Source
E. coli
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Tag
N- His-SUMO
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P52701
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Expression Region
1-400aa
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Molecular Weight
60.1 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
MSH6 is a key component of the DNA mismatch repair (MMR) system, which is essential for maintaining genomic stability by correcting erroneous insertions, deletions, and misincorporations that occur during DNA replication. Mutations in the MSH6 gene can lead to MSH6 deficiency, increasing susceptibility to various cancers, particularly Lynch syndrome-related tumors such as colorectal and endometrial cancers. Research on recombinant MSH6 protein has gained traction as scientists aim to understand its structural and functional dynamics within the MMR pathway. Through the production and characterization of recombinantly expressed MSH6, researchers can elucidate its interaction with other MMR proteins, such as MSH2, and gain insights into how MSH6 mutations compromise DNA repair processes. Additionally, studying the biochemical properties of MSH6 can inform therapeutic approaches, potentially leading to novel cancer treatments that target MMR deficiencies. The recombinant protein serves as a valuable tool in developing diagnostic assays and therapeutic strategies, highlighting the importance of MSH6 in cancer biology and the potential to exploit its pathways for clinical benefits. Overall, the exploration of MSH6 as a recombinant protein provides a critical window into understanding its role in cancer predisposition and the broader implications of MMR in genomic integrity.
