Analytical Data
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基因名
Frizzled-10/CD350
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简介
The Frizzled-10/CD350 gene is a member of the Frizzled gene family, encoding a 7-transmembrane domain protein that acts as a receptor for the Wingless-type MMTV integration site family signaling protein. Most Frizzled receptors, including Frizzled-10/CD350, are involved in cell signaling, specifically the β-catenin classical pathway. Frizzled-10/CD350 Protein, Human (HEK293, His) is the recombinant human-derived Frizzled-10/CD350 protein, expressed by HEK293 , with C-His labeled tag.
- Application
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生物活性
Measured by its ability to bind biotinylated recombinant mouse Wnt-3a in a functional ELISA. The ED50 for this effect is 4.336 nM. Measured by its ability to bind biotinylated recombinant mouse Wnt-3a in a functional ELISA. The ED50 for this effect is 4.336nM.
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别名
CD350; Frizzled homolog 10 (Drosophila); Frizzled-10; FZ-10
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种属
Human
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表达系统
HEK293
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标签
C-6*His
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纯度
Greater than 95% as determined by SDS-PAGE.
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蛋白编号
NP_009128.1
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表达区间
I21-G161
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蛋白长度
Partial
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分子量
20-25 kDa.
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Frizzled-10 (Fz10), also known as CD350, is a member of the Frizzled family of receptors, which are key players in the Wnt signaling pathway, involved in various developmental and physiological processes. This receptor is primarily known for its role in regulating cell proliferation, differentiation, and polarity during embryonic development. Research has shown that Fz10 interacts with Wnt ligands, activating downstream signaling cascades that influence cellular behavior. Importantly, abnormalities in Wnt signaling have been implicated in various diseases, including cancer and neurodegenerative disorders, making Fz10 a potential target for therapeutic interventions. The study of Fz10 as a recombinant protein has gained considerable attention, as it provides a platform for understanding its structure-function relationship, the dynamics of its interactions with Wnt proteins, and its regulatory mechanisms in cellular contexts. Additionally, the production of recombinant Fz10 enables the development of novel assays and tools for investigating its biological functions and the impact of various mutations associated with disease. As research advances, elucidating the role of Fz10/CD350 in both normal and pathological states could lead to significant insights into the treatment of Wnt-related diseases, thereby underscoring the importance of this protein in biomedical research.
