Analytical Data
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基因名
Frizzled-4/CD344
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简介
The Frizzled-4/CD344 protein is a receptor for Wnt proteins and is associated with the classical β-catenin pathway, activating disheveled proteins, inhibiting GSK-3 kinase, and triggering Wnt target genes. In retinal vascularization, it acts as a receptor for Wnt proteins and Norrin (NDP), stimulating β-catenin accumulation and LEF/TCF-mediated transcription. Frizzled-4/CD344 Protein, Human (HEK293, His) is the recombinant human-derived Frizzled-4/CD344 protein, expressed by HEK293 , with C-His labeled tag.
- Application
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生物活性
Measured by its binding ability in a functional ELISA. Immobilized Human Frizzled-4, at 0.1 μg/mL (100 μL/well) can bind Wnt-5a. The ED50 for this effect is 26.59 ng/mL. Measured by its binding ability in a functional ELISA. Immobilized Human Frizzled-4, at 0.1 μg/mL (100 μL/well) can bind Wnt-5a. The ED50 for this effect is 26.59 ng/mL.
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别名
CD344 antigen; CD344; EVR1; FEVR; Frizzled-4; Fz-4; FZD4
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种属
Human
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表达系统
HEK293
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标签
C-His
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纯度
Greater than 95% as determined by SDS-PAGE.
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蛋白编号
Q9ULV1
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表达区间
F37-E180
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蛋白长度
Partial
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分子量
22-25 kDa due to glycosylation
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Frizzled-4 (Fzd4), a member of the Frizzled family of G protein-coupled receptors, is known for its crucial role in the Wnt signaling pathway, which is pivotal in various developmental processes, including angiogenesis and neuronal differentiation. The significance of Fzd4 is particularly evident in the context of the vascular system; mutations in the Fzd4 gene have been linked to ocular and vascular diseases, such as familial exudative vitreoretinopathy (FEVR). Research on Fzd4/CD344 recombinant proteins has gained momentum as scientists seek to understand the mechanisms by which this receptor regulates cellular communication and tissue development. By generating and studying recombinant Fzd4 proteins, researchers can investigate the receptor’s ligand-binding properties, signaling mechanisms, and interactions with other cellular components, thus providing insights into its functional roles in health and disease. Given the potential therapeutic implications of modulating Wnt signaling, studies involving Fzd4/CD344 recombinant proteins could pave the way for novel interventions in disorders associated with aberrant angiogenesis and development.
