Analytical Data
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Gene name
NKX2-1
- Application
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Alternative Names
Homeobox protein NK-2 homolog A (Thyroid nuclear factor 1) (Thyroid transcription factor 1) (TTF-1) (Thyroid-specific enhancer-binding protein) (T/EBP) (NKX2A) (TITF1) (TTF1)
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Species
Human
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Source
E. coli
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Tag
N- His & C- Myc
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P43699
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Expression Region
1-371aa
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Molecular Weight
46.0 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
NKX2-1, also known as thyroid transcription factor-1 (TTF-1), is a vital homeobox gene that plays a crucial role in the development and function of various organs, particularly the thyroid, lung, and brain. Its importance is underscored by its involvement in the regulation of several key biological processes, including cell differentiation, organogenesis, and endocrine function. Mutations or dysregulation of NKX2-1 are linked to a range of developmental disorders and diseases, including congenital hypothyroidism, respiratory distress, and neurodevelopmental issues. Furthermore, NKX2-1 has been implicated as an oncogene in certain types of lung cancer, making it a target of interest in cancer research. The study of NKX2-1 recombinant protein allows researchers to investigate its structural and functional properties, enabling a better understanding of its molecular mechanisms and interaction with other factors involved in gene regulation. Additionally, the recombinant protein can serve as a valuable tool for the development of therapeutic strategies, including gene therapy and personalized medicine. Recent advances in recombinant protein technology have facilitated the production and purification of NKX2-1, paving the way for in-depth studies on its function and potential applications in both developmental biology and oncology.
