Analytical Data
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Gene name
ORC4
- Application
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Alternative Names
Origin recognition complex; subunit 4; S. cerevisiae; homolog of; FLJ46668; HSORC4; ORC 4; ORC 4L; ORC 4P; ORC4; ORC4_HUMAN; ORC4L; ORC4L protein; ORC4P; Origin recognition complex subunit 4 (yeast homolog) like; Origin recognition complex subunit 4; Origin recognition complex subunit 4 like (yeast); Origin recognition complex subunit 4 like; origin recognition complex; subunit 4 homolog; Origin recognition complex; subunit 4; S. cerevisiae; homolog-like
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Species
Human
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Source
E. coli
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Tag
N- His-SUMO
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
O43929
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Expression Region
1-436aa
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Molecular Weight
66.4 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
ORC4 protein is a crucial component of the origin recognition complex (ORC), which plays a significant role in the initiation of DNA replication in eukaryotic cells. As a part of a multi-subunit complex, ORC4 is responsible for recognizing and binding to the DNA at replication origins, thus facilitating the recruitment of additional factors necessary for the unwinding and synthesis of DNA. Research into ORC4 is particularly important due to its involvement in cell cycle regulation and its potential implications in cancer biology, as abnormal function or expression of replication machinery components can lead to genomic instability. Studies have shown that ORC4 interacts with various other proteins and regulatory elements, influencing processes beyond DNA replication, including chromatin remodeling and gene expression. Furthermore, understanding the structure and function of ORC4 at a molecular level can provide insights into the mechanisms governing DNA replication fidelity and regulation, which is critical for cellular health. Recent advances in biochemical and structural biology techniques have enabled more detailed investigations into the functional domains of ORC4 and its interactions, opening new avenues for therapeutic interventions targeting dysregulated replication mechanisms in cancers. Overall, the study of ORC4 and its role within the ORC represents a vital area of research that bridges molecular biology, genetics, and cancer therapeutics.
