Analytical Data
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基因名
xylE
- Application
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别名
CatO2ase Catechol 2,3-dioxygenase
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种属
Pseudomonas putida
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表达系统
E. coli
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标签
N- His-SUMO & C- Myc
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P06622
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表达区间
1-307aa
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分子量
55.2 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
The xylE gene encodes for a key enzyme in the degradation of aromatic compounds, specifically catechol, within the metabolic pathway of certain bacteria capable of utilizing aromatic compounds as carbon sources. This gene is often studied in the context of bioremediation and environmental microbiology, as its encoded protein plays a crucial role in breaking down toxic pollutants. The reconstruction of xylE protein allows researchers to explore its enzymatic properties, substrate specificity, and potential applications in bioremediation strategies. Given the growing environmental concerns related to aromatic hydrocarbons, such as those found in oil spills and industrial waste, understanding the function and mechanics of xylE becomes essential. Protein engineering and recombinant technology have facilitated the production of xylE protein in model organisms, enabling detailed biochemical characterization. Studying xylE and its recombinant variants enhances our knowledge of bacterial metabolism and can lead to the development of engineered microbial strains with improved capacities for pollutant degradation, thus contributing to sustainable environmental management practices. This research not only highlights the potential of microbial biocatalysts in detoxifying contaminated environments but also underscores the interrelationship between microbial activity and biotechnological advancements in solving environmental challenges.
