Analytical Data
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Gene name
MPK5
- Application
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Alternative Names
Benzothiadiazole-induced MAP kinase 1 MAP kinase 2 Multiple stress-responsive MAP kinase 2 OsBIMK1 OsMAP1 OsMAPK2 OsMAPK5 OsMPK3 OsMSRMK2 BIMK1, MAPK2, MAPK5, MPK3, MSRMK2
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Species
Oryza sativa subsp. japonica
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Source
E. coli
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Tag
N- His & C- Myc
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q10N20
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Expression Region
1-369aa
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Molecular Weight
48 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
The MPK5 protein, a member of the mitogen-activated protein kinase (MAPK) family, plays a crucial role in plant stress response and development. Research on MPK5 has gained attention due to its involvement in regulating various physiological processes, including disease resistance, environmental stress tolerance, and growth regulation. In particular, MPK5 is implicated in the plant's response to biotic stresses, such as pathogen attacks, where it helps modulate defense mechanisms. Studies have shown that MPK5 can be activated by various stimuli, leading to phosphorylation events that trigger downstream signaling pathways. This function makes it a vital component of the plant immune system. Furthermore, the exploration of MPK5 and its regulatory mechanisms can provide insights into improving crop resilience against environmental challenges and diseases. As global food security becomes increasingly threatened by climate change and agricultural diseases, understanding the role of MPK5 in plant biology presents significant potential for developing genetically engineered crops with enhanced stress tolerance and disease resistance. Thus, comprehensive studies on MPK5 and its interactions within the MAPK cascade are essential for advancing our knowledge in plant molecular biology and breeding strategies.
