Analytical Data
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基因名
CIDEA
- Application
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别名
CIDEA；Lipid transferase CIDEA
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
O60543
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表达区间
1-219aa
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氨基酸序列
MEAARDYAGA LIRPLTFMGS QTKRVLFTPL MHPARPFRVS NHDRSSRRGV MASSLQELIS KTLDALVIAT GLVTLVLEED GTVVDTEEFF QTLGDNTHFM ILEKGQKWMP GSQHVPTCSP PKRSGIARVT FDLYRLNPKD FIGCLNVKAT MYEMYSVSYD IRCTGLKGLL RSLLRFLSYS AQVTGQFLIY LGTYMLRVLD DKEERPSLRS QAKGRFTCG
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
CIDEA (Cell Death-Inducing DFFA-Like Effector A) is a member of the CIDE family of proteins, which play critical roles in various cellular processes, including apoptosis, lipid metabolism, and energy homeostasis. Originating from initial studies focused on programmed cell death, CIDEA has garnered interest due to its unique involvement in adipocyte function and fat storage regulation. Research indicates that CIDEA is highly expressed in white adipose tissue and serves as a vital regulator of lipid droplet dynamics, influencing lipid storage and mobilization. Its dysregulation has been linked to metabolic disorders, including obesity and insulin resistance, making it a potential target for therapeutic interventions. Furthermore, studies have shown that CIDEA interacts with other proteins involved in lipid metabolism, thereby affecting energy balance and overall metabolic homeostasis. Given the rising incidence of obesity and metabolic diseases worldwide, understanding CIDEA's functional mechanisms and regulatory pathways could provide significant insights for developing novel treatments aimed at combating these health issues. Consequently, ongoing efforts to explore the structural and functional properties of CIDEA, including its potential as a biomarker for metabolic health, are essential in the context of modern biomedical research.
