Analytical Data
-
Gene name
ATP1B1
- Application
-
Alternative Names
ATP1B1；ATP1B；Sodium/potassium-transporting ATPase subunit beta-1
-
Species
Human
-
Source
E. coli
-
Tag
His tag N-Terminus
-
Purity
Greater than 90% as determined by SDS-PAGE.
-
Uniprot
P05026
-
Expression Region
63-303aa
-
AA Sequence
ADPEFKPTYQ DRVAPPGLTQ IPQIQKTEIS FRPNDPKSYE AYVLNIVRFL EKYKDSAQRD DMIFEDCGDV PSEPKERGDF NHERGERKVC RFKLEWLGNC SGLNDETYGY KEGKPCIIIK LNRVLGFKPK PPKNESLETY PVMKYNPNVL PVQCTGKRDE DKDKVGNVEY FGLGNSPGFP LQYYPYYGKL LQPKYLQPLL AVQFTNLTMD TEIRIECKAY GENIGYSEKD RFQGRFDVKI EVKSHHHHHH
-
Molecular Weight
29 kDa
-
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
-
Form
Freeze-dried powder
-
Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
-
Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
ATP1B1, also known as the ATPase Na+/K+ transporting subunit beta 1, is a critical protein that plays a vital role in maintaining cellular ion balance by functioning as a component of the Na+/K+ ATPase pump. This enzyme regulates the concentration of sodium and potassium ions across the plasma membrane, which is essential for various cellular processes, including nutrient uptake, cell volume regulation, and electrical excitability in neurons and muscle cells. Mutations in the ATP1B1 gene have been associated with a range of pathologies, such as hypertension and neurological disorders, highlighting its importance in human health. Researchers have increasingly focused on characterizing recombinant forms of ATP1B1 to better understand its functional mechanisms and interactions with other cellular components, as well as its role in disease processes. The generation of recombinant ATP1B1 proteins allows for detailed studies of its biophysical properties, enzymatic activity, and response to pharmacological agents, which can lead to the identification of novel therapeutic targets. Further investigation into the mechanistic insights provided by recombinant ATP1B1 could pave the way for new treatment strategies for conditions linked to ion transport dysregulation, underscoring the relevance of this protein in both basic and applied biomedical research.
