Analytical Data
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基因名
GLB1
- Application
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别名
GLB1；ELNR1；Beta-galactosidase
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P16278
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表达区间
24-677aa
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氨基酸序列
NATQRMFEIDYSRDSFLKDGQPFRYISGSIHYSRVPRFYWKDRLLKMKMA GLNAIQTYVPWNFHEPWPGQYQFSEDHDVEYFLRLAHELGLLVILRPGPY ICAEWEMGGLPAWLLEKESILLRSSDPDYLAAVDKWLGVLLPKMKPLLYQ NGGPVITVQVENEYGSYFACDFDYLRFLQKRFRHHLGDDVVLFTTDGAHK TFLKCGALQGLYTTVDFGTGSNITDAFLSQRKCEPKGPLINSEFYTGWLD HWGQPHSTIKTEAVASSLYDILARGASVNLYMFIGGTNFAYWNGANSPYA AQPTSYDYDAPLSEAGDLTEKYFALRNIIQKFEKVPEGPIPPSTPKFAYG KVTLEKLKTVGAALDILCPSGPIKSLYPLTFIQVKQHYGFVLYRTTLPQD CSNPAPLSSPLNGVHDRAYVAVDGIPQGVLERNNVITLNITGKAGATLDL LVENMGRVNYGAYINDFKGLVSNLTLSSNILTDWTIFPLDTEDAVRSHLG GWGHRDSGHHDEAWAHNSSNYTLPAFYMGNFSIPSGIPDLPQDTFIQFPG WTKGQVWINGFNLGRYWPARGPQLTLFVPQHILMTSAPNTITVLELEWAP CSSDDPELCAVTFVDRPVIGSSVTYDHPSKPVEKRLMPPPPQKNKDSWLD HVVDHHHHHH
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分子量
74 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
GLB1, also known as beta-galactosidase, is an essential enzyme involved in the hydrolysis of beta-galactosidic bonds in various glycoconjugates, including glycoproteins and glycolipids. Its importance is underscored by its role in cellular metabolism and lysosomal function. Mutations in the GLB1 gene can lead to a spectrum of lysosomal storage disorders, notably GM1 gangliosidosis and Morquio B syndrome, which are characterized by the accumulation of GM1 gangliosides and other substances, resulting in severe neurological and systemic complications. The recombinant protein of GLB1 has garnered significant interest in biomedical research, particularly for enzyme replacement therapy. By producing GLB1 in a recombinant form, scientists aim to develop therapeutic strategies that could alleviate the clinical symptoms associated with GLB1 deficiency. Furthermore, understanding the three-dimensional structure and enzymatic mechanisms of GLB1 can provide insights into its functional role and the pathogenesis of related disorders. Current studies also explore various modifications of the recombinant protein to enhance its stability, activity, and delivery to target tissues. This research not only has implications for treatment but also enriches our understanding of enzyme function within the complex dynamics of lysosomal storage pathways. Overall, the investigation of GLB1 recombinant protein represents a promising avenue in the fields of enzyme therapy and molecular medicine, with the potential to significantly improve the quality of life for affected patients.
