Analytical Data
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基因名
FGFR-4
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简介
The FGFR-4 protein is a tyrosine-protein kinase receptor for fibroblast growth factor that regulates cellular processes, lipid metabolism, bile acid biosynthesis, and more.Its effects include downregulation of CYP7A1 in response to FGF19.FGFR-4 Protein, Rat (HEK293, C-hFc) is the recombinant rat-derived FGFR-4 protein, expressed by HEK293 , with C-hFc labeled tag.
- Application
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生物活性
1. Measured by its binding ability in a functional ELISA. 2. Immobilized human FGF18 at 10 μg/mL (100 μl/well) can bind Rat FGFR4 , The EC50 of Rat FGFR4 is <1.17 μg/mL. 3.Measured by its ability to inhibit FGF acidic-dependent proliferation of NIH-3T3 mouse fibroblast cells. The ED50 for this effect is 1.025 ng/mL, corresponding to a specific activity is 9.76×10^5 units/mg. Measured by its ability to inhibit FGF acidic-dependent proliferation of NIH-3T3 mouse fibroblast cells. The ED50 for this effect is 1.025 ng/mL, corresponding to a specific activity is 9.76×105 units/mg.
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别名
Fibroblast growth factor receptor 4; FGFR-4; CD334; JTK2; TKF
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种属
Rat
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表达系统
HEK293
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标签
C-hFc
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纯度
Greater than 95% as determined by reducing SDS-PAGE.
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蛋白编号
Q498D6
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表达区间
F17-D367
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氨基酸序列
FSLEASEEMEQEPCPAPISEQQEQVLTVALGQPVRLCCGRTERGRHWYKEGSRLASAGRVRGWRGRLEIASFLPEDAGRYLCLARGSMTVVHNLTLIMDDSLPSINNEDPKTLSSSSSGHSYLQQAPYWTHPQRMEKKLHAVPAGNTVKFRCPAAGNPMPTIHWLKNGQAFHGENRIGGIRLRHQHWSLVMESVVPSDRGTYTCLVENSLGSIRYSYLLDVLERSPHRPILQAGLPANTTAVVGSNVELLCKVYSDAQPHIQWLKHIVINGSSFGADGFPYVQVLKTTDINSSEVEVLYLRNVSAEDAGEYTCLAGNSIGLSYQSAWLTVLPAEEEDLAWTTATSEARYTD
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蛋白长度
Extracellular Domain
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分子量
110 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Fibroblast Growth Factor Receptor 4 (FGFR4) is a critical receptor involved in various biological processes such as embryonic development, cell proliferation, and differentiation. Abnormalities in FGFR4 signaling have been implicated in several types of cancers, including liver, breast, and prostate cancers, making it a significant target for therapeutic intervention. The study of FGFR4 recombinant proteins has gained momentum due to their potential utility in understanding receptor biology, developing monoclonal antibodies, and screening small-molecule inhibitors. Recombinant FGFR4 proteins can be produced in various expression systems, allowing for functional assays and structural studies that elucidate the receptor's role in disease. Recent research highlights the importance of FGFR4 in modulating tumor microenvironments and its crosstalk with other signaling pathways, underscoring the necessity for detailed investigations into its molecular mechanisms. By generating and characterizing FGFR4 recombinant proteins, researchers aim to advance the development of FGFR4-targeted therapies and diagnostic tools, ultimately contributing to more effective treatments for cancers associated with FGFR4 dysregulation.
