Analytical Data
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Gene name
SIAH1
- Application
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Alternative Names
Seven in absentia homolog 1
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Species
Human
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Source
E. coli
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Tag
N- GST
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q8IUQ4
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Expression Region
1-313aa
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Molecular Weight
61.6 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
SIAH1 (Seven In Absentia Homolog 1) is an E3 ubiquitin ligase that plays a crucial role in the regulation of various cellular processes, including apoptosis, cell cycle progression, and response to hypoxia. Its involvement in the ubiquitin-proteasome system allows SIAH1 to tag target proteins for degradation, thus maintaining cellular homeostasis. Research into SIAH1 has gained significant attention due to its implications in cancer biology and tumor progression, where dysregulation of SIAH1 has been associated with enhanced malignancy and resistance to therapy. Additionally, SIAH1 is known to interact with various oncogenes and tumor suppressor proteins, influencing signaling pathways that are critical for cell survival and proliferation. Consequently, studying SIAH1 and its associated pathways provides valuable insights into the molecular mechanisms underlying cancer development and progression, opening potential avenues for therapeutic interventions aimed at targeting SIAH1-related pathways. Furthermore, the production and characterization of recombinant SIAH1 protein have been pivotal in elucidating its biochemical properties and interactions, paving the way for the development of novel strategies to manipulate its activity in cancer treatment. Overall, the ongoing research on SIAH1 encompasses its structural biology, regulatory mechanisms, and its role in disease pathology, highlighting its significance as a target in cancer research and therapeutics.
