Analytical Data
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基因名
BRD2
- Application
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别名
BRD2;KIAA9001;RING3;Bromodomain-containing Protein 2
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P25440
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表达区间
65-187aa
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氨基酸序列
EVSNPKKPGRVTNQLQYLHKVVMKALWKHQFAWPFRQPVDAVKLGLPDYH KIIKQPMDMGTIKRRLENNYYWAASECMQDFNTMFTNCYIYNKPTDDIVL MAQTLEKIFLQKVASMPQEEQEL
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分子量
16 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
BRD2 (Bromodomain containing 2) is a member of the bromodomain and extraterminal (BET) protein family, which plays a crucial role in regulating gene expression through interaction with acetylated lysine residues on histones and non-histone proteins. This interaction is vital for various cellular processes, including transcriptional regulation, cell cycle progression, and differentiation. Dysregulation of BRD2 has been implicated in several diseases, notably cancer and inflammatory disorders. Studies have shown that BRD2 can influence the expression of oncogenes, thereby contributing to tumorigenesis. Due to its pivotal role in epigenetic regulation, BRD2 has emerged as a promising therapeutic target. Researchers are increasingly focusing on the development of BRD2 inhibitors to explore their potential in cancer treatment. Moreover, the production of recombinant BRD2 proteins is essential for elucidating its molecular mechanisms and interactions. By employing advanced protein expression systems, scientists aim to generate highly pure and functional BRD2 proteins that can be used in biochemical assays, structural studies, and drug discovery efforts. Understanding the structure-function relationship of BRD2 will facilitate the identification of specific inhibitors and enhance our overall comprehension of its role in health and disease. Thus, the study of BRD2, particularly through the lens of recombinant protein research, is crucial for advancing therapeutic strategies aimed at modulating its activity in various pathological conditions.












