Analytical Data
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Gene name
CERK
- Application
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Alternative Names
CERK；KIAA1646；Ceramide kinase
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q8TCT0
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Expression Region
1-537aa
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AA Sequence
MGATGAAEPLQSVLWVKQQRCAVSLEPARALLRWWRSPGPGAGAPGADAC SVPVSEIIAVEETDVHGKHQGSGKWQKMEKPYAFTVHCVKRARRHRWKWA QVTFWCPEEQLCHLWLQTLREMLEKLTSRPKHLLVFINPFGGKGQGKRIY ERKVAPLFTLASITTDIIVTEHANQAKETLYEINIDKYDGIVCVGGDGMF SEVLHGLIGRTQRSAGVDQNHPRAVLVPSSLRIGIIPAGSTDCVCYSTVG TSDAETSALHIVVGDSLAMDVSSVHHNSTLLRYSVSLLGYGFYGDIIKDS EKKRWLGLARYDFSGLKTFLSHHCYEGTVSFLPAQHTVGSPRDRKPCRAG CFVCRQSKQQLEEEQKKALYGLEAAEDVEEWQVVCGKFLAINATNMSCAC RRSPRGLSPAAHLGDGSSDLILIRKCSRFNFLRFLIRHTNQQDQFDFTFV EVYRVKKFQFTSKHMEDEDSDLKEGGKKRFGHICSSHPSCCCTVSNSSWN CDGEVLHSPAIEVRVHCQLVRLFARGIEENPKPDSHS
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Molecular Weight
60 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
CERK (chitinase-like domain-containing protein) is an important protein that plays a crucial role in plant defense and immune responses against pathogens. Research on CERK and its recombinant forms has gained significant attention due to their potential applications in biotechnology and agriculture. The protein is known to recognize chitin, a component of fungal cell walls, and subsequently activate defense mechanisms in plants, such as the production of reactive oxygen species and the expression of pathogenesis-related genes. Understanding the structure and function of CERK is essential for developing crops with enhanced resistance to diseases, which is particularly relevant in the context of global food security and sustainable agriculture. Recent studies have focused on the recombinant expression of CERK to delve deeper into its biochemical properties and interaction mechanisms. By producing CERK proteins in heterologous systems, researchers aim to elucidate the signaling pathways activated during pathogen attacks and the molecular interactions involved in plant immunity. Furthermore, insights gained from recombinant CERK studies could facilitate the engineering of resistant crops and the development of novel biopesticides, thereby reducing reliance on chemical pesticides and promoting environmentally friendly farming practices. Ultimately, ongoing research on CERK and its recombinant forms holds promise for advancing our understanding of plant-pathogen interactions and enhancing crop resilience in an era of climate change and increasing agricultural challenges.
