Analytical Data
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Gene name
COTL1
- Application
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Alternative Names
COTL1；CLP;Coactosin-like Protein
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q14019
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Expression Region
1-142aa
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AA Sequence
MGSSHHHHHH SSGLVPRGSH MATKIDKEAC RAAYNLVRDD GSAVIWVTFK YDGSTIVPGE QGAEYQHFIQ QCTDDVRLFA FVRFTTGDAMSKRSKFALIT WIGENVSGLQ RAKTGTDKTL VKEVVQNFAK EFVISDRKEL EEDFIKSELK KAGGANYDAQ TE
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Molecular Weight
18 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
COTL1, or cytoplasmic localized protein containing an LRR (leucine-rich repeat), is a significant protein implicated in various cellular processes, particularly those related to cell structure and motility. Research into COTL1 has gained momentum due to its role in cytoskeletal dynamics and its influence on the organization of actin filaments, which are critical for maintaining cell shape and facilitating movement. Additionally, COTL1 has been associated with key signaling pathways that regulate cellular responses to environmental stimuli. Abnormal expression or mutations in COTL1 have been linked to several diseases, including cancer, where alterations in cytoskeletal integrity can contribute to tumor progression and metastasis. The study of COTL1 recombinant protein provides insights into its functional mechanisms, interactions with other cellular components, and potential therapeutic targets. Researchers are utilizing recombinant DNA technology to produce COTL1 in various expression systems, enabling detailed biochemical and biophysical characterization. This approach helps elucidate the protein's structure-function relationship and its role in cellular processes, paving the way for discovering potential interventions in conditions where COTL1 is dysregulated. Overall, the investigation of COTL1 and its recombinant forms is crucial for understanding fundamental biological processes and developing strategies to mitigate diseases associated with its dysfunction.
