Analytical Data
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基因名
spoT
- Application
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别名
(p)((p), (ATP:GTP 3'-pyrophosphotransferase)(Stringent response-like protein)(ppGpp synthase II)(diphosphate), (Penta-phosphate guanosine-3'-pyrophosphohydrolase)((ppGpp)
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种属
Escherichia coli
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表达系统
E. coli
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标签
N- His & C- Myc
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P0AG24
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表达区间
1-702aa
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分子量
86.8 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
The research on spoT recombinant proteins is rooted in understanding the complex regulatory mechanisms of bacterial growth and stress responses, particularly in relation to the stringent response. SpoT is a bifunctional enzyme found in bacteria, responsible for synthesizing and hydrolyzing the second messenger molecule (p)ppGpp, which plays a crucial role in modulating gene expression during nutrient deprivation, environmental stress, and other physiological challenges. This regulatory pathway is vital for bacterial survival, as it helps the cells adapt to unfavorable conditions by altering metabolic processes, growth rates, and virulence factors. The study of spoT recombinant proteins helps to elucidate the enzyme's structure-function relationship and its role in the synthesis and degradation of (p)ppGpp, thus advancing our understanding of bacterial physiology. Furthermore, investigating spoT could have significant implications for antibiotic development, as targeting this pathway may provide new strategies to inhibit bacterial growth and combat antibiotic resistance. By generating and characterizing recombinant spoT proteins, researchers aim to provide insights into the enzyme's kinetic properties, regulatory mechanisms, and potential interactions with other cellular components, ultimately contributing to a deeper understanding of bacterial life cycles and their responses to stress.
