Analytical Data
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Gene name
DESI1
- Application
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Alternative Names
DESI1；FAM152B；PPPDE2;Desumoylating isopeptidase 1
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q6ICB0
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Expression Region
1-168aa
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AA Sequence
MGSSHHHHHH SSGLVPRGSH MGSMEPPNLY PVKLYVYDLS KGLARRLSPI MLGKQLEGIW HTSIVVHKDE FFFGSGGISS CPPGGTLLGP PDSVVDVGST EVTEEIFLEY LSSLGESLFR GEAYNLFEHN CNTFSNEVAQ FLTGRKIPSY ITDLPSEVLS TPFGQALRPL LDSIQIQPPG GSSVGRPNGQ S
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Molecular Weight
21 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
DESI1, or desumoylating isopeptidase 1, is a member of the isopeptidase family that plays a crucial role in the regulation of SUMO (Small Ubiquitin-like Modifier) protein modifications. These modifications are essential for various cellular processes, including signal transduction, DNA repair, and the regulation of gene expression. The study of DESI1 has gained significant attention due to its involvement in various diseases, particularly cancer, where altered SUMOylation patterns can lead to tumorigenesis and cancer progression. The ability of DESI1 to remove SUMO moieties from target proteins suggests that it acts as a regulatory switch, impacting protein function and cellular signaling pathways. Moreover, understanding the specific substrates of DESI1 and its mechanism of action could uncover potential therapeutic targets for SUMO-related diseases. Recent advances in structural biology have enabled researchers to characterize DESI1 at the molecular level, providing insights into its enzymatic activity and substrate recognition. The investigation of DESI1 not only enhances our understanding of the SUMO pathway but also contributes to the broader field of post-translational modifications, which are critical for maintaining cellular homeostasis and function. Consequently, the research on DESI1 recombinant protein has significant implications for developing innovative strategies to manipulate SUMOylation in disease contexts and offers potential avenues for novel therapeutic interventions.
