Analytical Data
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Gene name
rpsK
- Application
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Alternative Names
rpsK；Small ribosomal subunit Protein uS11
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Species
E.coli
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P0A7R9
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Expression Region
2-129aa
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AA Sequence
AKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGWATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNAAGFRITNITDVTPIPHNGCRPPKKRRV
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Molecular Weight
17.7 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
RpsK is a crucial ribosomal protein that plays a significant role in the assembly and function of the ribosomal subunit in bacteria. It is part of the 30S ribosomal subunit and is essential for protein synthesis, influencing the fidelity of translation and stability of the ribosome. The study of RpsK, particularly as a recombinant protein, has garnered attention due to its potential implications in understanding bacterial physiology and the mechanisms of antibiotic resistance. Recombinant RpsK can be produced through genetic engineering techniques, allowing for detailed structural and functional analyses. This research is critical in unraveling the intricate processes of bacterial translation, which can lead to the identification of new drug targets. Furthermore, with the rise of multi-drug resistant bacterial strains, a deeper understanding of RpsK could offer insights that contribute to the development of novel antibiotics or therapeutic strategies. By characterizing RpsK and its interactions within the ribosomal assembly, researchers aim to bridge gaps in knowledge regarding ribosomal function and its regulation, ultimately aiding the fight against bacterial infections.
