Analytical Data
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Gene name
PIEZO1
- Application
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Alternative Names
(Membrane protein induced by beta-amyloid treatment)(Mib)(Protein FAM38A)
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Species
Human
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Source
E. coli
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Tag
N- His & C- Myc
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q92508
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Expression Region
2198-2431aa
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Molecular Weight
33.6 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
PIEZO1, a mechanically activated ion channel, plays a crucial role in various physiological processes, including touch sensation, proprioception, and vascular function. It was first identified in 2010 through a functional screening approach, revealing its sensitivity to mechanical stimuli. The channel's distinct structure, characterized by its large, flexible extracellular domain, enables it to respond to mechanical forces such as shear stress and membrane deformation. Research has shown that PIEZO1 is involved in numerous biological functions, from regulating red blood cell volume to influencing the development of various tissues. Due to its significance in mechanotransduction, dysregulation of PIEZO1 has been linked to multiple diseases, including vascular disorders and anemia. Consequently, the reconstruction and functional characterization of PIEZO1 protein have become a focal point of scientific inquiry. This enables the study of its biophysical properties, ion selectivity, and mechanisms of activation under different mechanical forces. The recombinant PIEZO1 protein serves as a valuable tool for unraveling the channel's role in health and disease, paving the way for potential therapeutic interventions targeting PIEZO1-mediated pathways. Understanding its function at the molecular level could lead to advancements in treating conditions associated with mechanosensitive ion channels, thus highlighting the importance of PIEZO1 in both basic and applied biomedical research.
