Analytical Data
-
Gene name
ABCB1
- Application
-
Alternative Names
ABCB1；MDR1；PGY1；ATP-dependent translocase ABCB1
-
Species
Human
-
Source
E. coli
-
Tag
His tag N-Terminus
-
Purity
Greater than 90% as determined by SDS-PAGE.
-
Uniprot
P08183
-
Expression Region
350-710aa
-
AA Sequence
PSIEAFANARGAAYEIFKIIDNKPSIDSYSKSGHKPDNIKGNLEFRNVHFSYPSRKEVKILKGLNLKVQSGQTVALVGNSGCGKSTTVQLMQRLYDPTEGMVSVDGQDIRTINVRFLREIIGVVSQEPVLFATTIAENIRYGRENVTMDEIEKAVKEANAYDFIMKLPHKFDTLVGERGAQLSGGQKQRIAIARALVRNPKILLLDEATSALDTESEAVVQVALDKARKGRTTIVIAHRLSTVRNADVIAGFDDGVIVEKGNHDELMKEKGIYFKLVTMQTAGNEVELENAADESKSEIDALEMSSNDSRSSLIRKRSTRRSVRGSQAQDRKLSTKEALDESIPPVSFWRIMKLNLTEWPY
-
Molecular Weight
44.2 kDa
-
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
-
Form
Freeze-dried powder
-
Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
-
Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
ABCB1, also known as P-glycoprotein, is a crucial membrane protein that functions as an ATP-dependent efflux pump for various substrates, including drugs and xenobiotics. Its role in multidrug resistance (MDR) in cancer cells has garnered significant attention, as overexpression of ABCB1 is often linked to the failure of chemotherapy. Understanding the structure and function of ABCB1 is essential for unraveling the mechanisms underlying drug resistance and improving therapeutic strategies. The recombinant production of ABCB1 has been pursued to facilitate detailed studies of its biochemical properties, interactions with ligands, and potential inhibitors. By utilizing recombinant DNA technology, researchers aim to express and purify ABCB1 in a more controlled environment, allowing for high-resolution structural analysis through techniques such as X-ray crystallography and cryo-electron microscopy. This research not only provides insights into the ATP-binding and transport mechanisms of ABCB1 but also aids in the identification of compounds that can overcome drug resistance in cancer therapy. Moreover, studying ABCB1 can enhance our understanding of pharmacokinetics and drug-drug interactions, which is crucial for developing personalized medicine approaches. Overall, the exploration of recombinant ABCB1 is pivotal in the ongoing quest to tackle MDR and improve treatment outcomes for patients with various malignancies.
