Analytical Data
-
Gene name
mglB
- Application
-
Alternative Names
mglB;D-galactose/methyl-galactoside binding periplasmic Protein MglB
-
Species
E.coli
-
Source
E. coli
-
Tag
His tag N-Terminus
-
Purity
Greater than 90% as determined by SDS-PAGE.
-
Uniprot
P0AEE5
-
Expression Region
1-332aa
-
AA Sequence
MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVRKAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEKARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNKDGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAWLSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGTVLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK
-
Molecular Weight
35.7 kDa
-
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
-
Form
Freeze-dried powder
-
Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
-
Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
MglB is a key regulatory protein involved in the chemotactic response of various bacteria, particularly in the context of signal transduction pathways that govern motility and behavior in response to environmental stimuli. Research into the MglB protein has gained significant attention due to its crucial role in modulating bacterial chemotaxis, which is essential for survival and adaptation in diverse habitats. The understanding of MglB's function is further enhanced by its interaction with other proteins, such as CheW and CheA, which are integral to the chemotactic signaling cascade. Studies indicate that MglB acts as a molecular switch that transmits signals from the sensory to the response systems, thereby influencing flagellar movement and directionality. The recombinant expression of MglB protein allows for detailed structural and functional analyses, providing insights into its mechanism of action and potential applications in biotechnology and medicine. By utilizing techniques such as site-directed mutagenesis and crystallography, researchers aim to unravel the intricate details of MglB's structure and its dynamic role in bacterial chemotaxis. The implications of this research extend beyond fundamental microbial physiology, as exploring MglB's regulatory mechanisms could inform strategies for controlling pathogenic bacteria and developing novel therapeutic approaches. Overall, the study of MglB recombinant protein represents a significant frontier in microbiological research with broad potential applications in health and industry.











