Analytical Data
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Gene name
ERCC1
- Application
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Alternative Names
ERCC1;DNA excision repair Protein ERCC-1
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P07992
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Expression Region
1-273aa
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AA Sequence
MGSSHHHHHH SSGLVPRGSH MGSHMDPGKD KEGVPQPSGP PARKKFVIPL DEDEVPPGVA KPLFRSTQSL PTVDTSAQAA PQTYAEYAIS QPLEGAGATC PTGSEPLAGE TPNQALKPGA KSNSIIVSPR QRGNPVLKFV RNVPWEFGDV IPDYVLGQST CALFLSLRYH NLHPDYIHGR LQSLGKNFAL RVLLVQVDVK DPQQALKELA KMCILADCTL ILAWSPEEAG RYLETYKAYE QKPADLLMEK LEQDFVSRSL EQLIAASRED LALCPGLGPQ KARRLFDVLH EPFLKVP
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Molecular Weight
33 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
ERCC1 (Excision Repair Cross-Complementing Protein 1) is a critical protein involved in the DNA repair process, specifically in the nucleotide excision repair (NER) pathway. NER is essential for the removal of a variety of DNA lesions, including those induced by ultraviolet (UV) light and other environmental insults. The ERCC1 protein functions in partnership with another protein, XPF, forming a complex essential for the cleavage of damaged DNA. Mutations or deficiencies in ERCC1 are associated with a range of disorders, including cancer predisposition and neurodegenerative diseases, highlighting its role in maintaining genomic stability. Given its pivotal function in DNA repair, ERCC1 has become a focal point in cancer research, particularly concerning its implications for chemotherapy response. Elevated levels of ERCC1 have been correlated with resistance to platinum-based drugs in various cancers, making it a potential biomarker for treatment outcomes. Consequently, understanding the molecular mechanisms regulating ERCC1 expression and activity can inform strategies to enhance the efficacy of cancer therapies and improve patient prognoses. Researchers are increasingly exploring ERCC1 not only as a therapeutic target but also for its potential role in personalized medicine, where tailoring treatments based on the ERCC1 status of tumors could lead to more effective interventions. As our understanding of the complexities of DNA repair pathways deepens, the significance of ERCC1 in both basic and translational research continues to grow, making it a promising candidate for future studies aimed at combating cancer and other diseases linked to DNA damage.
