Analytical Data
-
基因名
NSD3
- Application
-
别名
NSD3；WHSC1L1；Histone-lysine N-methyltransferase NSD3
-
种属
Human
-
表达系统
E. coli
-
标签
His tag N-Terminus
-
纯度
Greater than 90% as determined by SDS-PAGE.
-
蛋白编号
Q9BZ95
-
表达区间
1021-1322aa
-
氨基酸序列
MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL EFPNLPYYI DGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGA VLDIRYGVSRIAYSKDFETLK VDFLSKLPEMLKMFEDRLCHKTYLNGD HVTHPDFMLYDALDVVLYMDPMCLDAFPKLV CFKKRIEAIPQIDKYLK SSKYIAWPLQGWQATFGGGDHPPKSDLEVLFQGPLGSEGDKS FAEGQT SINKTFKKALEEAAKRFQELKAQRESKEALEIEKNSRKPPPYKHIKANKV IG KVQIQVADLSEIPRCNCKPADENPCGLESECLNRMLQYECHPQVCP AGDRCQNQCF TKRLYPDAEIIKTERRGWGLRTKRSIKKGEFVNEYVGE LIDEEECRLRIKRAHENSVTN FYMLTVTKDRIIDAGPKGNYSRFMNHS CNPNCETQKWTVNGDVRVGLFALCDIPAGM ELTFNYNLDCLGNGRTEC HCGADNCSGFLGVRPKSACASTNEEKAKNAKLKQKRRK IKTEPKQMHE D
-
分子量
61 kDa
-
内毒素
< 1.0 EU per μg protein as determined by the LAL method.
-
性状
Freeze-dried powder
-
缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
-
稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Recombinant Human GPR141 Protein，His
Cat：PA2000-8039
Recombinant E.coli BAM1 Protein，His
Cat：PA2000-3851
Protein Description
NSD3 (Nuclear receptor-binding SET domain protein 3) is a member of the NSD family of histone methyltransferases, which play critical roles in gene regulation, cell differentiation, and development. Recent studies have highlighted NSD3's significant involvement in various biological processes, including the maintenance of pluripotency in stem cells and the regulation of oncogene expression in certain cancers. Aberrant expression or mutations of NSD3 have been linked to multiple malignancies, such as acute myeloid leukemia and glioblastoma, suggesting its potential as a therapeutic target. The reconstitution of NSD3 in vitro facilitates extensive analyses of its enzymatic activity, substrate specificity, and interaction with other epigenetic modifiers, providing insights into the molecular mechanisms governing its role in gene transcription and chromatin remodeling. Understanding the functional implications of NSD3, therefore, holds promise for uncovering novel pathways in cancer biology and for the development of targeted therapies aimed at disrupting its function in tumors where it is overexpressed or mutated. As a result, NSD3 has emerged as a focal point in epigenetic research, underscoring the need for further exploration of its biological significance and the potential for intervention in NSD3-related pathologies.
