Analytical Data
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Gene name
GPR50
- Application
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Alternative Names
GPR50; Melatonin-related receptor; G protein-coupled receptor 50; H9
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Species
Human
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Source
E. coli
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Tag
GST-tag at N-terminal
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q13585
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Expression Region
1-617aa
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AA Sequence
MGPTLAVPTPYGCIGCKLPQPEYPPALIIFMFCAMVITIVVDLIGNSMVILAVTKNKKLRNSGNIFVVSLSVADMLVAIYPYPLMLHAMSIGGWDLSQLQCQMVGFITGLSVVGSIFNIVAIAINRYCYICHSLQYERIFSVRNTCIYLVITWIMTVLAVLPNMYIGTIEYDPRTYTCIFNYLNNPVFTVTIVCIHFVLPLLIVGFCYVRIWTKVLAARDPAGQNPDNQLAEVRNFLTMFVIFLLFAVCWCPINVLTVLVAVSPKEMAGKIPNWLYLAAYFIAYFNSCLNAVIYGLLNENFRREYWTIFHAMRHPIIFFSGLISDIREMQEARTLARARAHARDQAREQDRAHACPAVEETPMNVRNVPLPGDAAAGHPDRASGHPKPHSRSSSAYRKSASTHHKSVFSHSKAASGHLKPVSGHSKPASGHPKSATVYPKPASVHFKADSVHFKGDSVHFKPDSVHFKPASSNPKPITGHHVSAGSHSKSAFSAATSHPKPTTGHIKPATSHAEPTTADYPKPATTSHPKPTAADNPELSASHCPEIPAIAHPVSDDSDLPESASSPAAGPTKPAASQLESDTIADLPDPTVVTTSTNDYHDVVVVDVEDDPDEMAV
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Molecular Weight
93.8 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
GPR50, a member of the G protein-coupled receptor family, has garnered significant attention due to its potential role in various physiological processes and its implications in metabolic and neuropsychiatric disorders. Emerging studies have suggested that GPR50 may influence circadian rhythms, energy metabolism, and mood regulation, linking it to conditions such as obesity, depression, and sleep disorders. The receptor exhibits unique characteristics, including its ability to form heterodimers with other receptors, which could modulate their functions and signaling pathways. Given its involvement in critical biological processes, research into GPR50 has focused on the characterization and functional analysis of its recombinant protein. This involves expressing GPR50 in heterologous systems to study its pharmacological properties, ligand binding, and downstream signaling mechanisms. Understanding GPR50's structure-function relationship and its interactions with ligands and other proteins is vital for elucidating its role in health and disease. Additionally, the development of specific GPR50 antagonists or agonists could provide novel therapeutic avenues for treating related disorders. Overall, the study of GPR50 recombinant protein is pivotal in advancing our knowledge of this enigmatic receptor and its potential as a target for drug discovery.
