Analytical Data
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Gene name
UBC10
- Application
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Alternative Names
UBC10；ubcD10；Ubiquitin-conjugating enzyme E2-18 kDa
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Species
E.coli
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P35133
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Expression Region
1-148aa
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AA Sequence
MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSESPYAGGVFLVTIHFPPDYPFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKTDKNKYESTARSWTQKYAMG
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Molecular Weight
20.6 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
UBC10 is a crucial protein involved in the ubiquitin-proteasome system, a key regulatory mechanism in cellular processes such as protein degradation, cell cycle progression, and response to stress. It plays a significant role in the tagging of substrates for degradation by conjugating ubiquitin, a small protein that marks other proteins for recycling or processing. Research on UBC10 has gained prominence due to its implications in various diseases, including cancer, where dysregulation of protein degradation pathways can lead to uncontrolled cell proliferation. Additionally, UBC10 has been implicated in the regulation of immune responses and in the maintenance of cellular homeostasis. Understanding the structure and function of UBC10, as well as the mechanisms by which it interacts with other cellular proteins, is essential for exploring potential therapeutic targets. Recent studies have focused on the reconstitution of UBC10 in vitro to elucidate its functional domains, improve the understanding of its enzymatic activity, and provide insights into its role in pathophysiological conditions. This research not only enhances our knowledge of the ubiquitin system but also paves the way for the development of novel drugs that can manipulate UBC10 activity for therapeutic benefits.
