Analytical Data
-
基因名
HECTD3
- Application
-
别名
E3 ubiquitin-protein ligase HECTD3; HECD3_HUMAN; HECT; HECT domain containing protein 3; HECT domain-containing protein 3; HECTD3; Probable E3 ubiquitin protein ligase HECTD3
-
种属
Human
-
表达系统
E. coli
-
标签
GST-tag at N-terminal
-
纯度
Greater than 90% as determined by SDS-PAGE.
-
蛋白编号
Q5T447
-
表达区间
1-210aa
-
氨基酸序列
MEGMDKETFEFKFGKELTFTTVLSDQQVVELIPGGAGIVVGYGDRSRFIQLVQKARLEESKEQVAAMQAGLLKVVPQAVLDLLTWQELEKKVCGDPEVTVDALRKLTRFEDFEPSDSRVQYFWEALNNFTNEDRSRFLRFVTGRSRLPARIYIYPDKLGYETTDALPESSTCSSTLFLPHYASAKVCEEKLRYAAYNCVAIDTDMSPWEE
-
分子量
50.4 kDa
-
内毒素
< 1.0 EU per μg protein as determined by the LAL method.
-
性状
Freeze-dried powder
-
缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
-
稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Recombinant Human GCN5L2 Protein，His
Cat：PA1000-1212
Recombinant Human cry1Ia Protein，His
Cat：PA2000-4052
Protein Description
HECTD3 is a member of the HECT (Homologous to E6-AP Carboxyl Terminus) E3 ubiquitin ligase family, which plays a crucial role in the ubiquitin-proteasome system, responsible for regulating protein degradation and modulating various cellular processes. Research on HECTD3 has gained attention due to its potential involvement in diverse biological functions, including cell proliferation, differentiation, and apoptosis. Abnormalities in ubiquitin ligases like HECTD3 are linked to various diseases, including cancer, neurodegenerative disorders, and developmental abnormalities. The study of HECTD3 recombinant protein allows for the exploration of its specific enzymatic activity and substrate interactions, providing insights into its regulatory mechanisms. Furthermore, understanding the role of HECTD3 in the ubiquitin signaling pathway may reveal novel therapeutic targets for diseases associated with dysregulated protein homeostasis. Recent studies have suggested that HECTD3 may be implicated in cellular stress responses and pathways associated with tumorigenesis, emphasizing the need for further investigation into its molecular function and the implications of its dysregulation in disease contexts. Overall, the characterization of HECTD3 recombinant protein is essential for elucidating its biological roles and potential applications in targeted therapies.
