Analytical Data
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Gene name
GTF2B
- Application
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Alternative Names
GTF2B;TF2B;TFIIB;Transcription initiation factor IIB
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q00403
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Expression Region
1-316aa
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AA Sequence
MASTSRLDALPRVTCPNHPDAILVEDYRAGDMICPECGLVVGDRVIDVGSEWRTFSNDKATKDPSRVGDSQNPLLSDGDLSTMIGKGTGAASFDEFGNSKYQNRRTMSSSDRAMMNAFKEITTMADRINLPRNIVDRTNNLFKQVYEQKSLKGRANDAIASACLYIACRQEGVPRTFKEICAVSRISKKEIGRCFKLILKALETSVDLITTGDFMSRFCSNLCLPKQVQMAATHIARKAVELDLVPGRSPISVAAAAIYMASQASAEKRTQKEIGDIAGVADVTIRQSYRLIYPRAPDLFPTDFKFDTPVDKLPQL
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Molecular Weight
50.8kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
GTF2B (General Transcription Factor II B) is a crucial protein involved in the initiation of transcription by RNA polymerase II, playing a pivotal role in the assembly of the transcription machinery and regulation of gene expression. The interest in GTF2B recombinant protein studies stems from its significance in the transcriptional regulation process, where it acts as a cofactor that bridges the RNA polymerase II and the transcriptional complex. Dysfunctional GTF2B has been linked to various diseases, including certain cancers and developmental disorders. Recent research efforts have focused on the production of high-purity recombinant GTF2B, allowing for detailed biochemical and structural analysis. This enables scientists to investigate its interactions with other transcription factors, characterize its functional domains, and understand its regulatory mechanisms. Such studies are crucial for deciphering the complexities of gene expression and could pave the way for targeted therapeutic strategies aimed at diseases related to transcriptional misregulation. Furthermore, understanding GTF2B's role in the context of other transcription factors may reveal insights into its contribution to epigenetic modifications and long-range gene regulation. Overall, the characterization of GTF2B as a recombinant protein provides a foundational platform for advancing our knowledge of transcription mechanisms and their implications in health and disease.
