Analytical Data
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Gene name
HSD17B1
- Application
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Alternative Names
HSD17B1;E17KSR;EDH17B1;EDH17B2;17-beta-hydroxysteroid dehydrogenase type 1
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P14061
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Expression Region
1-328aa
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AA Sequence
MGSSHHHHHH SSGLVPRGSH MGSHMARTVV LITGCSSGIG LHLAVRLASD PSQSFKVYAT LRDLKTQGRL WEAARALACP PGSLETLQLD VRDSKSVAAA RERVTEGRVD VLVCNAGLGL LGPLEALGED AVASVLDVNV VGTVRMLQAF LPDMKRRGSG RVLVTGSVGG LMGLPFNDVY CASKFALEGL CESLAVLLLP FGVHLSLIEC GPVHTAFMEK VLGSPEEVLD RTDIHTFHRF YQYLAHSKQV FREAAQNPEE VAEVFLTALR APKPTLRYFT TERFLPLLRM RLDDPSGSNY VTAMHREVFG DVPAKAEAGA EAGGGAGPGA EDEAGRGAVG DPELGDPPAA PQ
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Molecular Weight
38 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
HSD17B1, or Hydroxysteroid 17-beta dehydrogenase type 1, is an enzyme that plays a critical role in the steroidogenic pathway, particularly in the conversion of estrone to estradiol, a key estrogen in the regulation of various physiological processes including reproduction, metabolism, and bone health. Abnormal expression or activity of HSD17B1 has been implicated in several endocrine disorders and various cancers, notably breast cancer. Given its significance in hormone metabolism, HSD17B1 has garnered attention as a potential therapeutic target, making the study of its recombinant protein crucial for understanding its structure-function relationship and developing inhibitors. Research on HSD17B1 recombinant protein involves the expression, purification, and characterization of this enzyme, which is essential for elucidating its enzymatic mechanisms and regulatory pathways. Insights gained from these studies can facilitate the identification of novel biomarkers and therapeutic strategies, particularly in hormone-related diseases. Furthermore, investigating variations in HSD17B1 activity due to genetic polymorphisms can enhance our understanding of individual susceptibility to hormonal imbalances and related health conditions. Overall, the exploration of HSD17B1 recombinant protein not only advances fundamental biological knowledge but also has significant implications for clinical applications in endocrinology and oncology.
