Cat: PA2000-4628

Recombinant Human shf Protein,His

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Analytical Data

  • Gene name

    shf

  • Application

    SPRMSTBLIITCELISACELL ASSAYDRUG SCREENING

  • Alternative Names

    shf;SH2 domain-containing adapter Protein F

  • Species

    Human

  • Source

    E. coli

  • Tag

    His tag N-Terminus

  • Purity

    Greater than 90% as determined by SDS-PAGE.

  • Uniprot

    Q7M4L6

  • Expression Region

    1-423aa

  • AA Sequence

    MQQEGGPVRS APCRTGTLEG SRQGSPGHRK RASPKGSLSS AQPHSWMLTP SPLNSHCAHR EPISSSPQPV ANGPKQKKKS NWRSTTRLRI IRLRDRLEPR PLAILEDYAD PFDVQETGEG SAGASGAPEK VPENDGYMEP YEAQKMMAEI RGSKETATQP LPLYDTPYEP EEDGATAEGE GAPWPRESRL PEDDERPPEE YDQPWEWKKE RISKAFAVDI KVIKDLPWPP PVGQLDSSPS LPDGDRDISG PASPLPEPSL EDSSAQFEGP EKSCLSPGRE EKGRLPPRLS AGNPKSAKPL SMEPSSPLGE WTDPALPLEN QVWYHGAISR TDAENLLRLC KEASYLVRNS ETSKNDFSLS LKSSQGFMHM KLSRTKEHKY VLGQNSPPFS SVPEIVHHYA SRKLPIKGAE HMSLLYPVAI RTL

  • Molecular Weight

    46.7 kDa

  • Endotoxin

    < 1.0 EU per μg protein as determined by the LAL method.

  • Form

    Freeze-dried powder

  • Buffer formulation

    PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.

  • Reconstitution

    Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.

  • Customization

    Site-directed mutagenesis Custom tag design Custom buffer formulation Custom full-length protein production

  • Stability Test

    The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.

  • Storage & Shelf Life

    Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

  • Shipping

    In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.

Quality inspection process

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Protein Description

The research on SHF (Sphingosine-1-Phosphate Receptor-1) recombinant proteins has garnered significant attention due to its potential implications in various physiological and pathological processes. SHF proteins play a critical role in cellular signaling, influencing pathways related to cell proliferation, migration, and survival. Abnormal SHF signaling has been implicated in a range of diseases, including cancer, cardiovascular disorders, and immune-related conditions. Understanding the functional mechanisms of SHF proteins at the molecular level can provide valuable insights into disease progression and offer novel therapeutic targets. The use of recombinant DNA technology enables the production of large quantities of purified SHF proteins, facilitating detailed biochemical and biophysical studies. Furthermore, recombinant SHF proteins serve as crucial tools for developing assays and screening compounds that can modulate their activity. As the field of protein engineering advances, exploring SHF recombinant proteins may lead to breakthroughs in therapeutic strategies, enhancing our ability to combat diseases associated with dysregulated sphingosine-1-phosphate signaling.

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