Analytical Data
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基因名
VD3
- Application
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别名
VD3;CYP1ALPHA;CYP27B;25-hydroxyvitamin D-1 alpha hydroxylase. mitochondrial
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
O15528
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表达区间
1-508aa
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氨基酸序列
MTQTLKYASRVFHRVRWAPELGASLGYREYHSARRSLADIPGPSTPSFLAELFCKGGLSRLHELQVQGAAHFGPVWLASFGTVRTVYVAAPALVEELLRQEGPRPERCSFSPWTEHRRCRQRACGLLTAEGEEWQRLRSLLAPLLLRPQAAARYAGTLNNVVCDLVRRLRRQRGRGTGPPALVRDVAGEFYKFGLEGIAAVLLGSRLGCLEAQVPPDTETFIRAVGSVFVSTLLTMAMPHWLRHLVPGPWGRLCRDWDQMFAFAQRHVERREAEAAMRNGGQPEKDLESGAHLTHFLFREELPAQSILGNVTELLLAGVDTVSNTLSWALYELSRHPEVQTALHSEITAALSPGSSAYPSATVLSQLPLLKAVVKEVLRLYPVVPGNSRVPDKDIHVGDYIIPKNTLVTLCHYATSRDPAQFPEPNSFRPARWLGEGPTPHPFASLPFGFGKRSCMGRRLAELELQMALAQILTHFEVQPEPGAAPVRPKTRTVLVPERSINLQFLDR
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分子量
56.5 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
VD3 recombinant protein, also known as vitamin D3 recombinant protein, has gained significant attention in the field of biomedical research due to its crucial role in calcium metabolism and immune system regulation. Vitamin D3, primarily synthesized in the skin through sunlight exposure, is essential for maintaining bone health and preventing diseases such as osteoporosis and rickets. However, many individuals suffer from vitamin D deficiency due to limited sun exposure, dietary insufficiency, or certain health conditions. The development of VD3 recombinant protein offers a promising avenue for therapeutic interventions, providing a controlled and consistent source of vitamin D3 for supplementation. This approach enables researchers to explore its effects on various physiological processes, including cellular differentiation, immune response, and potential anti-inflammatory properties. In recent years, studies have also investigated the implications of VD3 recombinant protein in the context of chronic diseases, such as diabetes, cardiovascular disorders, and autoimmunity, underscoring its potential as a versatile therapeutic agent. Moreover, the advancement of recombinant DNA technology has facilitated the production of this protein, ensuring higher purity and bioavailability compared to traditional supplementation methods. Overall, the research surrounding VD3 recombinant protein emphasizes its importance not only in addressing nutrient deficiencies but also in its potential role in enhancing health outcomes across different populations.












