Analytical Data
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Gene name
dNER
- Application
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Alternative Names
dNER；BET；Delta and Notch-like epidermal growth factor-related receptor
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q8NFT8
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Expression Region
1-640aa
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AA Sequence
MQPRRAQAPGAQLLPALALLLLLLGAGPRGSSLANPVPAAPLSAPGPCAAQPCRNGGVCTSRPEPDPQHPAPAGEPGYSCTCPAGISGANCQLVADPCASNPCHHGNCSSSSSSSSDGYLCICNEGYEGPNCEQALPSLPATGWTESMAPRQLQPVPATQEPDKILPRSQATVTLPTWQPKTGQKVVEMKWDQVEVIPDIACGNASSNSSAGGRLVSFEVPQNTSVKIRQDATASLILLWKVTATGFQQCSLIDGRSVTPLQASGGLVLLEEMLALGNNHFIGFVNDSVTKSIVALRLTLVVKVSTCVPGESHANDLECSGKGKCTTKPSEATFSCTCEEQYVGTFCEEYDACQRKPCQNNASCIDANEKQDGSNFTCVCLPGYTGELCQSKIDYCILDPCRNGATCISSLSGFTCQCPEGYFGSACEEKVDPCASSPCQNNGTCYVDGVHFTCNCSPGFTGPTCAQLIDFCALSPCAHGTCRSVGTSYKCLCDPGYHGLYCEEEYNECLSAPCLNAATCRDLVNGYECVCLAEYKGTHCELYKDPCANVSCLNGATCDSDGLNGTCICAPGFTGEECDIDINECDSNPCHHGGSCLDQPNGYNCHCPHGWVGANCEIHLQWKSGHMAESLTNMPRHSLY
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Molecular Weight
100 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
dNER (Drosophila Nucleotide Excision Repair) recombinant proteins are pivotal in the study of DNA repair mechanisms, particularly the nucleotide excision repair pathway, which is vital for maintaining genomic stability and preventing mutations. This pathway is responsible for the recognition and removal of a wide range of DNA lesions, including those caused by UV irradiation and chemical carcinogens. Understanding dNER proteins is essential, as they share significant functional similarities with human proteins involved in the same repair processes, offering insights into human genetic disorders resulting from defective DNA repair systems, such as xeroderma pigmentosum and cancer. Research on dNER recombinant proteins involves cloning, expression, and purification techniques to produce these proteins in model organisms like Drosophila. This allows scientists to study their interactions, enzymatic activities, and roles in the DNA repair process. Investigating these proteins also aids in elucidating the consequences of impaired nucleotide excision repair in organisms, serving as a model to better understand the underlying mechanisms in humans. By studying the structure-function relationships of dNER proteins, researchers aim to develop innovative therapeutic strategies to enhance DNA repair capabilities and mitigate the effects of DNA damage in various diseases. Thus, dNER recombinant protein research not only contributes to a foundational understanding of cellular defense mechanisms against DNA damage but also highlights its implications for health and disease management.
