Analytical Data
-
Gene name
ENTPD1
- Application
-
Alternative Names
ENTPD1；CD39；Ectonucleoside triphosphate diphosphohydrolase 1
-
Species
Human
-
Source
E. coli
-
Tag
His tag N-Terminus
-
Purity
Greater than 90% as determined by SDS-PAGE.
-
Uniprot
P49961
-
Expression Region
1-510aa
-
AA Sequence
MEDTKESNVKTFCSKNILAILGFSSIIAVIALLAVGLTQNKALPENVKYG IVLDAGSSHTSLYIYKWPAEKENDTGVVHQVEECRVKGPGISKFVQKVNE IGIYLTDCMERAREVIPRSQHQETPVYLGATAGMRLLRMESEELADRVLD VVERSLSNYPFDFQGARIITGQEEGAYGWITINYLLGKFSQKTRWFSIVP YETNNQETFGALDLGGASTQVTFVPQNQTIESPDNALQFRLYGKDYNVYT HSFLCYGKDQALWQKLAKDIQVASNEILRDPCFHPGYKKVVNVSDLYKTP CTKRFEMTLPFQQFEIQGIGNYQQCHQSILELFNTSYCPYSQCAFNGIFL PPLQGDFGAFSAFYFVMKFLNLTSEKVSQEKVTEMMKKFCAQPWEEIKTS YAGVKEKYLSEYCFSGTYILSLLLQGYHFTADSWEHIHFIGKIQGSDAGW TLGYMLNLTNMIPAEQPLSTPLSHSTYVFLMVLFSLVLFTVAIIGLLIFH KPSYFWKDMV
-
Molecular Weight
82 kDa
-
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
-
Form
Freeze-dried powder
-
Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
-
Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
ENTPD1, also known as ecto-nucleotide triphosphate diphosphohydrolase-1, is an enzyme that plays a crucial role in hydrolyzing extracellular nucleotides, including ATP and ADP, to their respective monophosphates. Its function is significant in regulating purinergic signaling, which is involved in various physiological processes such as cell proliferation, apoptosis, and immune responses. Dysregulation of ENTPD1 has been implicated in several diseases, including cancer, where it can influence tumor microenvironments and immune evasion. The study of recombinant ENTPD1 proteins facilitates the exploration of their enzymatic properties, substrate specificity, and potential as therapeutic targets. By producing and characterizing these recombinant proteins, researchers can better understand the enzyme's structure-function relationships and its role in pathophysiological contexts. This knowledge could lead to the development of ENTPD1 inhibitors that may serve as novel therapeutic agents in treating diseases characterized by altered purinergic signaling, thereby advancing our comprehension of its biological significance and potential for clinical applications.
