Analytical Data
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Gene name
MBD3
- Application
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Alternative Names
MBD3;Methyl-CpG-binding domain Protein 3
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
O95983
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Expression Region
1-291aa
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AA Sequence
MGSSHHHHHH SSGLVPRGSH MGSMERKRWE CPALPQGWER EEVPRRSGLS AGHRDVFYYS PSGKKFRSKP QLARYLGGSM DLSTFDFRTG KMLMSKMNKS RQRVRYDSSN QVKGKPDLNT ALPVRQTASI FKQPVTKITN HPSNKVKSDP QKAVDQPRQL FWEKKLSGLN AFDIAEELVK TMDLPKGLQG VGPGCTDETL LSAIASALHT STMPITGQLS AAVEKNPGVW LNTTQPLCKA FMVTDEDIRK QEELVQQVRK RLEEALMADM LAHVEELARD GEAPLDKACA EDDDEEDEEE EEEEPDPDPE MEHV
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Molecular Weight
35 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
MBD3 (Methyl-CpG-binding domain protein 3) is a crucial component of the multiprotein complexes that mediate gene repression through DNA methylation. Its role in the epigenetic regulation of gene expression has garnered significant interest, particularly with its involvement in neuronal differentiation and maintenance of pluripotency in stem cells. Research has shown that MBD3 can interact with various transcription factors and other chromatin-modifying proteins, contributing to the formation and function of the NuRD (Nucleosome Remodeling and Deacetylase) complex. This complex plays a pivotal role in remodeling chromatin structure, enhancing transcriptional silencing of target genes associated with development, cancer, and other cellular processes. Furthermore, mutations in MBD3 have been linked to neurodevelopmental disorders, highlighting its importance in brain function and development. As a result, the study of MBD3 recombinant proteins aims to further elucidate its structural and functional properties, paving the way for potential therapeutic strategies targeting epigenetic regulations in diseases. By investigating the mechanisms by which MBD3 influences gene expression and chromatin dynamics, researchers seek to understand its contributions to normal and pathological states, opening avenues for novel drug development and interventions in disorders characterized by epigenetic dysregulation.
