Analytical Data
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Gene name
METTL1
- Application
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Alternative Names
WDR4;tRNA (guanine-N(7)-)-methyltransferase non-catalytic subunit WDR4
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9UBP6
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Expression Region
1-276aa
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AA Sequence
MGSSHHHHHHSSGLVPRGSHMAAETRNVAGAEAPPPQKRYYRQRAHSNPM ADHTLRYPVKPEEMDWSELYPEFFAPLTQNQSHDDPKDKKEKRAQAQVEF ADIGCGYGGLLVELSPLFPDTLILGLEIRVKVSDYVQDRIRALRAAPAGG FQNIACLRSNAMKHLPNFFYKGQLTKMFFLFPDPHFKRTKHKWRIISPTL LAEYAYVLRVGGLVYTITDVLELHDWMCTHFEEHPLFERVPLEDLSEDPV VGHLGTSTEEGKKVLRNGGKNFPAIFRRIQDPVLQAVTSQTSLPGH
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Molecular Weight
34 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
METTL1 (Methyltransferase-like 1) is an emerging protein with critical roles in various biological processes, including RNA modification and regulation. As a member of the methyltransferase family, METTL1 is primarily involved in the methylation of adenosine residues in ribosomal RNA, which is essential for ribosome biogenesis and function. Its dysfunction has been implicated in several diseases, including cancer, where altered RNA methylation patterns may contribute to tumorigenesis. The need for METTL1 recombinant protein arises in the context of understanding its structure-function relationship and elucidating its role in cellular metabolism and gene expression. Given its potential as a therapeutic target, investigators are keen to produce and characterize METTL1 to explore its enzymatic activity, interaction with RNA substrates, and its regulatory mechanisms in various pathways. Thus, the production of METTL1 recombinant protein provides a vital tool for both basic research and potential therapeutic applications, further advancing our understanding of epitranscriptomics.
