Cat: PA1000-2444

Recombinant Human POLR1C Protein,His

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Analytical Data

  • Gene name

    POLR1C

  • Application

    SPRMSTBLIITCELISACELL ASSAYDRUG SCREENING

  • Alternative Names

    POLR1C;POLR1E;DNA-directed RNA polymerases I and III subunit RPAC1

  • Species

    Human

  • Source

    E. coli

  • Tag

    His tag N-Terminus

  • Purity

    Greater than 90% as determined by SDS-PAGE.

  • Uniprot

    O15160

  • Expression Region

    1-346aa

  • AA Sequence

    MGSSHHHHHH SSGLVPRGSH MGS MAASQAVEEM RSRVVLGEFG VRNVHTTDFP GNYSGYDDAW DQDRFEKNFR VDVVHMDENS LEFDMVGIDA AIANAFRRIL LAEVPTMAVE KVLVYNNTSI VQDEILAHRL GLIPIHADPR LFEYRNQGDE EGTEIDTLQF RLQVRCTRNP HAAKDSSDPN ELYVNHKVYT RHMTWIPLGN QADLFPEGTI RPVHDDILIA QLRPGQEIDL LMHCVKGIGK DHAKFSPVAT ASYRLLPDIT LLEPVEGEAA EELSRCFSPG VIEVQEVQGK KVARVANPRL DTFSREIFRN EKLKKVVRLA RVRDHYIFSV ESTGVLPPDV LVSEAIKVLM GKCRRFLDEL DAVQMD

  • Molecular Weight

    42 kDa

  • Endotoxin

    < 1.0 EU per μg protein as determined by the LAL method.

  • Form

    Freeze-dried powder

  • Buffer formulation

    PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.

  • Reconstitution

    Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.

  • Customization

    Site-directed mutagenesis Custom tag design Custom buffer formulation Custom full-length protein production

  • Stability Test

    The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.

  • Storage & Shelf Life

    Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

  • Shipping

    In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.

Quality inspection process

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Protein Description

POLR1C, a critical component of RNA polymerase I, plays a vital role in the transcription of ribosomal RNA (rRNA), which is essential for ribosome biogenesis and consequently for cellular protein synthesis. Research on POLR1C has gained significant attention due to its involvement in various cellular processes and its implications in diseases, including cancer. Mutations or dysregulation of POLR1C can lead to defects in rRNA synthesis, resulting in impaired ribosome production and cellular growth, which highlights its importance in maintaining normal cellular functions. In recent years, studies have demonstrated that POLR1C is not only crucial for ribosomal RNA transcription but also participates in the regulation of other cellular pathways, making it a potential target for therapeutic interventions. Understanding the structural and functional properties of recombinant POLR1C is essential for elucidating its role in transcription regulation and its contribution to the pathogenesis of related diseases. This ongoing research holds promise for advancing knowledge regarding gene expression regulation and may inform the development of novel strategies for treating diseases linked to POLR1C dysfunction.

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