Analytical Data
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Gene name
EIF2B2
- Application
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Alternative Names
EIF2B2；EIF2BB；Translation initiation factor eIF2B subunit beta
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P49770
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Expression Region
1-351aa
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AA Sequence
MPGSAAKGSELSERIESFVETLKRGGGPRSSEEMARETLGLLRQIITDHRWSNAGELMELIRREGRRMTAAQPSETTVGNMVRRVLKIIREEYGRLHGRSDESDQQESLHKLLTSGGLNEDFSFHYAQLQSNIIEAINELLVELEGTMENIAAQALEHIHSNEVIMTIGFSRTVEAFLKEAARKRKFHVIVAECAPFCQGHEMAVNLSKAGIETTVMTDAAIFAVMSRVNKVIIGTKTILANGALRAVTGTHTLALAAKHHSTPLIVCAPMFKLSPQFPNEEDSFHKFVAPEEVLPFTEGDILEKVSVHCPVFDYVPPELITLFISNIGGNAPSYIYRLMSELYHPDDHVL
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Molecular Weight
66.0kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Eukaryotic translation initiation factor 2B (EIF2B) is a critical multi-subunit complex that plays a vital role in protein synthesis by facilitating the exchange of GDP for GTP on the initiation factor EIF2, thereby promoting the formation of the translation initiation complex. Among its five subunits, EIF2B2 is particularly noteworthy due to its implications in various cellular processes, including cell growth, differentiation, and stress responses. Mutations in EIF2B2 and other EIF2B subunits have been linked to neurological disorders, notably hypomyelinating leukodystrophy, a condition characterized by impaired myelin formation in the nervous system. This has propelled the scientific community's interest in understanding the functional mechanisms and structural properties of EIF2B2. Recent advances in recombinant protein technology allow researchers to produce and purify EIF2B2 in a laboratory setting, paving the way for detailed biochemical and biophysical studies. By elucidating the structure-function relationship of EIF2B2, scientists aim to uncover its regulatory mechanisms during translation initiation and its role in disease pathways. Such research not only contributes to our fundamental understanding of protein synthesis but may also inform the development of therapeutic strategies for disorders associated with EIF2B dysfunction.
