Analytical Data
-
基因名
EIF2C4
- Application
-
别名
EIF2C4；EIF2C4；KIAA1567；Protein argonaute-4
-
种属
Human
-
表达系统
E. coli
-
标签
His tag N-Terminus
-
纯度
Greater than 90% as determined by SDS-PAGE.
-
蛋白编号
Q9HCK5
-
表达区间
1-861aa
-
氨基酸序列
MEALGPGPPASLFQPPRRPGLGTVGKPIRLLANHFQVQIPKIDVYHYDVD IKPEKRPRRVNREVVDTMVRHFKMQIFGDRQPGYDGKRNMYTAHPLPIGR DRVDMEVTLPGEGKDQTFKVSVQWVSVVSLQLLLEALAGHLNEVPDDSVQ ALDVITRHLPSMRYTPVGRSFFSPPEGYYHPLGGGREVWFGFHQSVRPAM WNMMLNIDVSATAFYRAQPIIEFMCEVLDIQNINEQTKPLTDSQRVKFTK EIRGLKVEVTHCGQMKRKYRVCNVTRRPASHQTFPLQLENGQAMECTVAQ YFKQKYSLQLKYPHLPCLQVGQEQKHTYLPLEVCNIVAGQRCIKKLTDNQ TSTMIKATARSAPDRQEEISRLVKSNSMVGGPDPYLKEFGIVVHNEMTEL TGRVLPAPMLQYGGRNKTVATPNQGVWDMRGKQFYAGIEIKVWAVACFAP QKQCREDLLKSFTDQLRKISKDAGMPIQGQPCFCKYAQGADSVEPMFKHL KMTYVGLQLIVVILPGKTPVYAEVKRVGDTLLGMATQCVQVKNVVKTSPQ TLSNLCLKINAKLGGINNVLVPHQRPSVFQQPVIFLGADVTHPPAGDGKK PSIAAVVGSMDGHPSRYCATVRVQTSRQEISQELLYSQEVIQDLTNMVRE LLIQFYKSTRFKPTRIIYYRGGVSEGQMKQVAWPELIAIRKACISLEEDY RPGITYIVVQKRHHTRLFCADKTERVGKSGNVPAGTTVDSTITHPSEFDF YLCSHAGIQGTSRPSHYQVLWDDNCFTADELQLLTYQLCHTYVRCTRSVS IPAPAYYARLVAFRARYHLVDKDHDSAEGSHVSGQSNGRDPQALAKAVQI HHDTQHTMYFA
-
分子量
124 kDa
-
内毒素
< 1.0 EU per μg protein as determined by the LAL method.
-
性状
Freeze-dried powder
-
缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
-
稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Recombinant Human NKX2-8 Protein，His
Cat：PA2000-9724
Recombinant Human EIF2B2 Protein，His
Cat：PA2000-1148
Protein Description
EIF2C4, also known as Argonaute 4 (AGO4), is a crucial component of the RNA-induced silencing complex (RISC) and plays a significant role in the regulation of gene expression through RNA interference (RNAi) mechanisms. It is involved in the post-transcriptional regulation of gene silencing by binding to small RNAs, particularly microRNAs (miRNAs) and small interfering RNAs (siRNAs), and guiding them to target mRNAs for degradation or translational repression. The importance of EIF2C4 has been underscored by its involvement in various biological processes, including development, cell differentiation, and response to environmental stress. Additionally, aberrations in RNAi pathways, including those involving EIF2C4, have been linked to several diseases, including cancers and viral infections. Recent advances in biotechnology have allowed for the production of recombinant EIF2C4 protein, enabling researchers to study its structure, function, and interactions in detail. Understanding the molecular mechanisms underlying EIF2C4 activity could provide insights into the regulatory networks of gene expression and potentially reveal new therapeutic targets for diseases related to RNAi dysregulation. As RNAi-based therapies continue to evolve, the characterization of EIF2C4 and its role in gene silencing remains a focal point of molecular biology research, with implications for both fundamental science and clinical applications.
