Analytical Data
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基因名
FOXD3
- Application
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别名
FOXD3；HFH2；Forkhead box Protein D3
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q9UJU5
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表达区间
2-478aa
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氨基酸序列
29 amino acid Tag-GEFTLSGGGSASDMSGQTVLTAEDVDIDVVGEGDDGLEEKDSDAGC DSPAGPPEL RLDEADEVPPAAPHHGQPQPPHQQPLTLPKEAAGAGAGP GGDVGAPEADGCKGGVGGEEGGASG GGPGAGSGSAGGLAPSKPKNSLV KPPYSYIALITMAILQSPQKKLTLSGICEFISNRFPYYREK FPAWQNS IRHNLSLNDCFVKIPREPGNPGKGNYWTLDPQSEDMFDNGSFLRRRKRFK RHQQEHL REQTALMMQSFGAYSLAAAAGAAGPYGRPYGLHPAAAAGAY SHPAAAAAAAAAAALQYPYALPP VAPVLPPAVPLLPSGELGRKAAAFG SQLGPGLQLQLNSLGAAAAAAGTAGAAGTTASLIKSEPS ARPSFSIEN IIGGGPAAPGGSAVGAGVAGGTGGSGGGSTAQSFLRPPGTVQSAALMATH QPLSL SRTTATIAPILSVPLSGQFLQPAASAAAAAAAAAQAKWPAQLE ESGGGGSPGRRRRRRRRRRR
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分子量
48 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
FOXD3 (Forkhead box D3) is a member of the Forkhead transcription factor family, which plays a pivotal role in various biological processes, including embryogenesis, cell differentiation, and maintenance of pluripotency in stem cells. Its expression is notably linked to the regulation of key developmental pathways and has been implicated in the processes of neural crest formation and tumorigenesis. In recent years, research has intensified on FOXD3 due to its potential role as a biomarker for certain cancers and its involvement in maintaining stem cell characteristics, particularly in pluripotent stem cells. The production and study of FOXD3 recombinant protein have become essential for elucidating its molecular mechanisms and interactions. Recombinant FOXD3 can be utilized in various experimental settings, including in vitro assays to assess its function in transcriptional regulation, protein-protein interactions, and to investigate its role in inhibiting differentiation of pluripotent cells. Furthermore, understanding the structural and functional properties of FOXD3 through recombinant protein studies can provide critical insights into its involvement in developmental biology and pathology, paving the way for novel therapeutic strategies in regenerative medicine and cancer treatment. Overall, the study of FOXD3 recombinant protein is a crucial step towards deciphering its complex roles in cellular and developmental contexts, ultimately contributing to advancements in understanding stem cell biology and cancer research.
