Analytical Data
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基因名
EARS2
- Application
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别名
EARS2；KIAA1970；Nondiscriminating glutamyl-tRNA synthetase EARS2. mitochondrial
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q5JPH6
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表达区间
1-523aa
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氨基酸序列
MAALLRRLLQRERPSAASGRPVGRREANLGTDAGVAVRVRFAPSPTGFLHLGGLRTALYNYIFAKKYQGSFILRLEDTDQTRVVPGAAENIEDMLEWAGIPPDESPRRGGPAGPYQQSQRLELYAQATEALLKTGAAYPCFCSPQRLELLKKEALRNHQTPRYDNRCRNMSQEQVAQKLAKDPKPAIRFRLEQVVPAFQDLVYGWNRHEVASVEGDPVIMKSDGFPTYHLACVVDDHHMGISHVLRGSEWLVSTAKHLLLYQALGWQPPHFAHLPLLLNRDGSKLSKRQGDVFLEHFAADGFLPDSLLDIITNCGSGFAENQMGRTLPELITQFNLTQVTCHSALLDLEKLPEFNRLHLQRLVSNESQRRQLVGKLQVLVEEAFGCQLQNRDVLNPVYVERILLLRQGHICRLQDLVSPVYSYLWTRPAVGRAQLDAISEKVDVIAKRVLGLLERSSMSLTQDMLNGELKKLSEGLEGTKYSNVMKLLRMALSGQQQGPPVAEMMLALGPKEVRERIQKVVSS
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分子量
58.6 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
EARS2, or mitochondrial glutamyl-tRNA synthetase, plays a crucial role in mitochondrial protein synthesis by catalyzing the attachment of glutamate to its corresponding tRNA, which is essential for the translation of mitochondrial mRNAs. Mutations in the EARS2 gene have been linked to various mitochondrial disorders, including leukodystrophies and other neurodegenerative diseases, underscoring its importance in cellular function and development. The study of EARS2 recombinant proteins provides valuable insights into its enzymatic mechanisms, structural characteristics, and interactions with other mitochondrial components. With the rising prevalence of mitochondrial diseases, understanding the biochemistry and functional implications of EARS2 is imperative for developing targeted therapeutic strategies. By employing recombinant DNA techniques, researchers can produce and purify EARS2, facilitate detailed biochemical assays, and investigate its role in mitochondrial translation and related pathways. Additionally, such studies may shed light on the pathophysiology associated with EARS2 mutations, paving the way for innovative treatment approaches and interventions. Thus, through the investigation of EARS2 recombinant proteins, researchers aim to elucidate the molecular basis of mitochondrial function and disease, contributing to the broader field of mitochondrial biology and medicine.
