Analytical Data
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基因名
ARHGEF10
- Application
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别名
ARHGA_HUMAN; ARHGEF10; DKFZp686H0726; GEF10
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种属
Human
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表达系统
E. coli
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标签
GST-tag at N-terminal
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
O15013
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表达区间
1-380aa
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氨基酸序列
MENPEEAIYDDVPRENSDSEPDEMIYDDVENGDEGGNSSLEYGWSSSEFESYEEQSDSECKNGIPRSFLRSNHKKQMQKLVKAAKDGTKDGLERTRAAVKRGRSFIRTKSLIAQDHRSSLEEEQNLFIDVDCKHPEAILTPMPEGLSQQQVVRRYILGSVVDSEKNYVDALKRILEQYEKPLSEMEPKVLSERKLKTVFYRVKEILQCHSLFQIALASRVSEWDSVEMIGVVFVASFSKSMVLDAYSEYVNNFSTAVAVLKKTCATKPAFLEFLKQEQEASPDRTTLYSLMMKPIQRFPQFILLLQDMLKNTSKGHPDRLPLQMALTELETLAEKLNERKRDADQRCEVKQIAKAINERYLNKVERGFLQLYSKIIFALC
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分子量
70.1 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
ARHGEF10 (Rho guanine nucleotide exchange factor 10) is a member of the Rho GEF family, which plays a crucial role in regulating the activity of Rho GTPases—key players in various cellular processes such as cytoskeletal dynamics, cell migration, and signal transduction. Dysregulation of Rho GTPases has been implicated in numerous diseases, including cancer, neurological disorders, and cardiovascular diseases. Research on ARHGEF10 has gained traction due to its potential role in these pathological conditions, particularly in the context of neuronal development and axon guidance. Specific mutations in the ARHGEF10 gene have been linked to peripheral neuropathies, highlighting its importance in maintaining neuronal integrity. The recombinant protein expression of ARHGEF10 allows for detailed studies of its biochemical properties, interactions, and regulatory mechanisms. Understanding its function could pave the way for therapeutic strategies targeting Rho GTPase signaling pathways, offering insights into novel treatment options for related diseases. Thus, the production and characterization of ARHGEF10 recombinant protein remain a critical avenue of research to elucidate its role in health and disease.
