Analytical Data
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基因名
PAPD1
- Application
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别名
0610027A18Rik; AW551379; EC 2.7.7.19; FLJ10486; mitochondrial; Mitochondrial poly(A) polymerase; mtPAP; PAP; PAP-associated domain-containing protein 1; PAPD1; PAPD1_HUMAN; Poly(A) RNA polymerase; Poly(A) RNA polymerase. mitochondrial; Poly(A) RNA polymerase. mitochondrial precursor; Polynucleotide adenylyltransferase; RP11-305E6.3; Terminal uridylyltransferase 1; TUTase 1
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q9NVV4
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表达区间
38-582 aa
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氨基酸序列
KDL RRDEQPSGSV ETGFEDKIPK RRFSEMQNER REQAQRTVLI HCPEKISENK FLKYLSQFGP INNHFFYESF GLYAVVEFCQ KESIGSLQNG THTPSTAMET AIPFRSRFFN LKLKNQTSER SRVRSSNQLP RSNKQLFELL CYAESIDDQL NTLLKEFQLT EENTKLRYLT CSLIEDMAAA YFPDCIVRPF GSSVNTFGKL GCDLDMFLDL DETRNLSAHK ISGNFLMEFQ VKNVPSERIA TQKILSVLGE CLDHFGPGCV GVQKILNARC PLVRFSHQAS GFQCDLTTNN RIALTSSELL YIYGALDSRV RALVFSVRCW ARAHSLTSSI PGAWITNFSL TMMVIFFLQR RSPPILPTLD SLKTLADAED KCVIEGNNCT FVRDLSRIKP SQNTETLELL LKEFFEYFGN FAFDKNSINI RQGREQNKPD SSPLYIQNPF ETSLNISKNV SQSQLQKFVD LARESAWILQ QEDTDRPSIS SNRPWGLVSL LLPSAPNRKS FTKKKSNKFA IETVKNLLES LKGNRTENFT KTSGKRTIST QT
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分子量
66.1 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
PAPD1, or Polyadenylate Polymerase Domain Containing 1, is a protein that plays a crucial role in RNA metabolism, particularly in the polyadenylation process, which involves the addition of a poly(A) tail to mRNA molecules. This modification is essential for mRNA stability, export from the nucleus, and efficient translation. Recent studies have highlighted the importance of PAPD1 in various cellular processes, including gene regulation, response to cellular stress, and involvement in disease states, such as cancer and neurological disorders. Research into PAPD1 is driven by its potential as a therapeutic target, given its significant role in modulating gene expression and RNA processing. Moreover, understanding the mechanistic functions of PAPD1 could provide insights into the complex regulatory networks governing cellular activity. Recombining PAPD1 for biochemical studies allows for detailed analysis of its interaction with other proteins and RNA substrates, advancing our knowledge about its operational pathways and implications in health and disease. Such insights may pave the way for developing novel strategies to manipulate RNA metabolism for therapeutic benefit.
