Analytical Data
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Gene name
PAPD4
- Application
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Alternative Names
TENT2; GLD2; PAPD4Poly(A) RNA polymerase GLD2; hGLD-2; EC 2.7.7.19; PAP-associated domain-containing protein 4; Terminal nucleotidyltransferase 2; Terminal uridylyltransferase 2; TUTase 2
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q6PIY7
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Expression Region
1-484 aa
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AA Sequence
MFPNSILGRP PFTPNHQQHN NFFTLSPTVY SHQQLIDAQF NFQNADLSRA VSLQQLTYGN VSPIQTSASP LFRGRKRLSD EKNLPLDGKR QRFHSPHQEP TVVNQIVPLS GERRYSMPPL FHTHYVPDIV RCVPPFREIA FLEPREITLP EAKDKLSQQI LELFETCQQQ ISDLKKKELC RTQLQREIQL LFPQSRLFLV GSSLNGFGTR SSDGDLCLVV KEEPCFFQVN QKTEARHILT LVHKHFCTRL SGYIERPQLI RAKVPIVKFR DKVSCVEFDL NVNNIVGIRN TFLLRTYAYL ENRVRPLVLV IKKWASHHQI NDASRGTLSS YSLVLMVLHY LQTLPEPILP SLQKIYPESF SPAIQLHLVH QAPCNVPPYL SKNESNLGDL LLGFLKYYAT EFDWNSQMIS VREAKAIPRP DGIEWRNKYI CVEEPFDGTN TARAVHEKQK FDMIKDQFLK SWHRLKNKRD LNSILPVRAA VLKR
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Molecular Weight
56.0 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
PAPD4, or poly(A) polymerase delta 4, is a member of the poly(A) polymerase family that plays a crucial role in RNA metabolism, particularly in the polyadenylation of RNA transcripts. The importance of polyadenylation in the regulation of gene expression, mRNA stability, and translation efficiency underscores the significance of studying PAPD4. Recent research has highlighted its involvement in various cellular processes, including stress responses and developmental regulation. Abnormal expression or dysfunction of PAPD4 has been linked to several diseases, including cancer and neurological disorders, indicating its potential as a therapeutic target. Given the increasing interest in the post-transcriptional regulation of genes, understanding the structure, function, and interactions of PAPD4 is essential for elucidating its biological roles and therapeutic potentials. Current studies focus on characterizing the recombinant PAPD4 protein to provide insights into its enzymatic activity, substrate specificity, and regulatory mechanisms, paving the way for developing novel strategies in disease treatment and RNA-based therapies.
